Journal Article

A drug-mediated organic electrochemical transistor for robustly reusable biosensors

Zou, Ye, Ma, Yingqiao, Zhang, Fengjiao, Xiong, Qi, Wang, Shu, Zhi, Danfeng, Yang, Junfang, Ye, Dekai, Xiang, Lanyi, Jiang, Ziling, Peng, Qian, Li, Jia, Dai, Xiaojuan, Di, Chong-an, He, Zihan

2024

Overview

Reusable point-of-care biosensors offer a cost-effective solution for serial biomarker monitoring, addressing the critical demand for tumour treatments and recurrence diagnosis. However, their realization has been limited by the contradictory requirements of robust reusability and high sensing capability to multiple interactions among transducer surface, sensing probes and target analytes. Here we propose a drug-mediated organic electrochemical transistor as a robust, reusable epidermal growth factor receptor sensor with striking sensitivity and selectivity. By electrostatically adsorbing protonated gefitinib onto poly(3,4-ethylenedioxythiophene):polystyrene sulfonate and leveraging its strong binding to the epidermal growth factor receptor target, the device operates with a unique refresh-in-sensing mechanism. It not only yields an ultralow limit-of-detection concentration down to 5.74 fg ml −1 for epidermal growth factor receptor but, more importantly, also produces an unprecedented regeneration cycle exceeding 200. We further validate the potential of our devices for easy-to-use biomedical applications by creating an 8 × 12 diagnostic drug-mediated organic electrochemical transistor array with excellent uniformity to clinical blood samples. Both reusability and sensing capability of biosensors are required but usually challenging to achieve. Here a reusable and highly sensitive epidermal growth factor receptor sensor based on a drug-mediated organic electrochemical transistor is reported.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 119/118 / 140/133 / 140/146 / 639/301/1005/1007 / 639/301/1005/1009 / Biomarkers / Biomaterials / Biomedical materials / Biosensing Techniques - instrumentation / Biosensors / Bridged Bicyclo Compounds, Heterocyclic - chemistry / Chemistry and Materials Science / Condensed Matter Physics / Electrochemical Techniques - instrumentation / Electrochemistry / Epidermal growth factor / ErbB Receptors / Growth factors / Humans / Inhibitor drugs / Materials Science / Nanotechnology / Optical and Electronic Materials / Polymers - chemistry / Polystyrene resins / Polystyrenes - chemistry / Receptors / Robustness (mathematics) / Sensitivity analysis / Sensor arrays / Transistors / Transistors, Electronic