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From Lost in Translation to Paradise Found: Enabling Protein Biomarker Method Transfer by Mass Spectrometry
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From Lost in Translation to Paradise Found: Enabling Protein Biomarker Method Transfer by Mass Spectrometry
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Journal Article
From Lost in Translation to Paradise Found: Enabling Protein Biomarker Method Transfer by Mass Spectrometry
2014
Overview
[...]LC-MS/MS-based clinical protein analysis has predominantly focused on improved analytical measurement for well-established biomarkers (5). Postextraction stability should be determined for both pools after storage in the autosampler (^24 h, reinjecting aliquots if feasible), freezing (^72 h, if rou- tine), and extract freeze-thaw for 1 and 2 cycles. Because many preclinical studies rely on biobanked materials, it should be noted that at least 3 freshly acquired samples should be evaluated for stability of 1 freeze-thaw cycle (assay fresh, freeze for ^12 h, thaw for ^2 h, reassay, and compare).
