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Comparison of various semen extenders and addition of prostaglandin F2α on pregnancy rate in cows
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Comparison of various semen extenders and addition of prostaglandin F2α on pregnancy rate in cows
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Journal Article
Comparison of various semen extenders and addition of prostaglandin F2α on pregnancy rate in cows
2016
Overview
This investigation comprises three trials. Trial 1 consists of an in vitro comparison of three semen extenders: two egg yolk based (customized Tris-egg yolk-glycerol and Triladyl®), the third (AndroMed®) soybean lecithin based. With regard to post-thaw motility, the phytoextender AndroMed® proved to be superior (59±3% v. 53±2% and 53±2%, P<0.05). It had earlier been shown that addition of the commercial prostaglandin F2α
preparation Dinolytic® before freezing compromises post-thaw motility; therefore, in Trial 2, Dinolytic® was added after thawing. Frozen-thawed spermatozoa tolerated addition of Dinolytic® at a concentration of 30% (v/v). In Trial 3, cows were inseminated using straws in which diluted semen and Dinolytic® were frozen in the same straw, separated by an air bubble, so intermingling could only take place in the course of insemination. Pregnancy rates at Dinolytic® dosages of 0%, 30% or 60% amounted to 44%, 41% and 56%, respectively (P>0.05), a result that encourages a large-scale field study, which is envisioned.
Publisher
Cambridge University Press,Elsevier B.V,Elsevier Limited,Elsevier
Subject
/ Alcohol
/ Animals
/ Cattle
/ cows
/ Cryopreservation - veterinary
/ Dinoprost - administration & dosage
/ Egg Yolk
/ Female
/ Freezing
/ Glycerol
/ Insemination, Artificial - veterinary
/ Lecithin
/ Male
/ Motility
/ Nitrogen
/ Physiology and functional biology of systems
/ Semen
/ Semen Preservation - veterinary
/ Soybeans
/ Sperm
/ Straw
/ Thawing
/ Yolk
