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Srs2 prevents Rad51 filament formation by repetitive motion on DNA
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Journal Article
Srs2 prevents Rad51 filament formation by repetitive motion on DNA
2013
Overview
Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by Srs2. Rad51 forms filaments on single-stranded DNA by sequential binding of primarily monomers and dimers in a 5′–3′ direction. One Rad51 molecule binds to three nucleotides, and six monomers are required to achieve a stable nucleation cluster. Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. The same activity of Srs2 cannot prevent RecA filament formation, indicating its specificity for Rad51. Srs2’s DNA-unwinding activity is greatly suppressed when Rad51 filaments form on duplex DNA. Taken together, our results reveal an exquisite and highly specific mechanism by which Srs2 regulates the Rad51 filament formation.
Srs2 is a DNA helicase and single-stranded DNA translocase that prevents homologous recombination by dismantling Rad51 filaments. Qiu
et al.
use single-molecule techniques to describe Rad51 filament formation and show that Srs2 displays repetitive activity on single-stranded DNA, which prevents re-formation of Rad51 filaments after dismantling.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Adenosine Triphosphate - metabolism
/ DNA
/ DNA, Single-Stranded - genetics
/ DNA, Single-Stranded - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Homologous Recombination - genetics
/ Humanities and Social Sciences
/ Rad51 Recombinase - biosynthesis
/ Rad51 Recombinase - genetics
/ Rec A Recombinases - biosynthesis
/ Rec A Recombinases - genetics
/ Rec A Recombinases - metabolism
/ Saccharomyces cerevisiae - genetics
/ Saccharomyces cerevisiae Proteins - biosynthesis
/ Saccharomyces cerevisiae Proteins - genetics
/ Saccharomyces cerevisiae Proteins - metabolism
/ Science
