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CcGSDMEa functions the pore-formation in cytomembrane and the regulation on the secretion of IL-lβ in common carp (Cyprinus carpio haematopterus)
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CcGSDMEa functions the pore-formation in cytomembrane and the regulation on the secretion of IL-lβ in common carp (Cyprinus carpio haematopterus)
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CcGSDMEa functions the pore-formation in cytomembrane and the regulation on the secretion of IL-lβ in common carp (Cyprinus carpio haematopterus)
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Journal Article
CcGSDMEa functions the pore-formation in cytomembrane and the regulation on the secretion of IL-lβ in common carp (Cyprinus carpio haematopterus)
2023
Overview
GSDME is the only direct executor of caspase-dependent pyroptosis in both canonical and non-canonical inflammasomes known to date in fish, and plays an important role in anti-bacterial infection and inflammatory response. In order to determine the regulation of GSDMEa on antibacterial infection in innate immune response, the CcGSDMEa gene in common carp ( Cyprinus carpio haematopterus ) was first identified and characterized, and then its function related to immune defense was investigated. Our results showed that the expressions of Cc GSDMEa at the mRNA and protein levels were both significantly increased after Aeromonas hydrophila intraperitoneal infection at the early stage than that in the control group. We found that Cc GSDMEa could be cleaved by inflammatory caspase ( Cc Caspase-1b) and apoptotic caspases ( Cc Caspase-3a/b and Cc Caspase-7a/b). Interestingly, only the Cc GSDMEa-NT (1-252 aa) displayed bactericidal activity to Escherichia coli and could punch holes in the membrane of HEK293T cells, whereas Cc GSDMEa-FL (1-532 aa) and Cc GSDMEa-CT (257-532 aa) showed no above activity and pore-forming ability. Overexpression of Cc GSDMEa increased the secretion of Cc IL-1β and the release of LDH, and could reduce the A. hydrophila burdens in fish. On the contrary, knockdown of Cc GSDMEa reduced the secretion of Cc IL-1β and the release of LDH, and could increase the A. hydrophila burdens in fish. Taken together, the elevated expression of Cc GSDMEa was a positive immune response to A. hydrophila challenge in fish. Cc GSDMEa could perform the pore-formation in cell membrane and the regulation on the secretion of IL-lβ, and further regulate the bacterial clearance in vivo . These results suggested that Cc GSDMEa played an important role in immune defense against A. hydrophila and could provide a new insight into understanding the immune mechanism to resist pathogen invasion in teleost.
