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Nanopore sensors for nucleic acid analysis
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Journal Article
Nanopore sensors for nucleic acid analysis
2011
Overview
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
Recent advances suggest that nanopore-based sensors may be able to sequence the human genome for under $1,000. This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Acids
/ Animals
/ Biosensing Techniques - instrumentation
/ Biosensing Techniques - methods
/ Biosensing Techniques - trends
/ Chemistry and Materials Science
/ DNA
/ DNA, Single-Stranded - analysis
/ Genetics
/ Genomes
/ Humans
/ Nanotechnology and Microengineering
/ Polymers
/ Sensors
/ Sequence Analysis, DNA - instrumentation
/ Sequence Analysis, DNA - methods
/ Sequence Analysis, DNA - trends
/ Velocity
