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![In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey](https://www.mbrl.ae/o/mbrl-theme/images/site-assets/generic/no-book-image.png)
In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey
by
Chan, Kim Wei
, Shafiq Cheng, Mohamad Zulhafiz
, Mohd Lila, Mohd Azmi
, Nur-Fazila, Saulol Hamid
, Khalifa, Shaden A M
, El-Seedi, Hesham R
, Zulkhairi Amin, Fatin Aina
, Ismail, Norsharina
, Sabri, Suriana
, Mohd Esa, Norhaizan
, Zawawi, Norhasnida
in
Abnormalities
/ Acute toxicity
/ Animals
/ Bacillus amyloliquefaciens
/ Bacteria
/ Bees
/ Biochemical tests
/ Biochemistry
/ Biocompatibility
/ Blood
/ Body Weight
/ Dosage
/ Female
/ functional foods
/ Glycerol
/ Hematology
/ Hemoglobin
/ Honey
/ In vivo methods and tests
/ Oral administration
/ Organs
/ probiotic safety
/ Probiotics
/ Rats
/ Rats, Sprague-Dawley
/ Sprague Dawley rat
/ Subacute toxicity
2023
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In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey
by
Chan, Kim Wei
, Shafiq Cheng, Mohamad Zulhafiz
, Mohd Lila, Mohd Azmi
, Nur-Fazila, Saulol Hamid
, Khalifa, Shaden A M
, El-Seedi, Hesham R
, Zulkhairi Amin, Fatin Aina
, Ismail, Norsharina
, Sabri, Suriana
, Mohd Esa, Norhaizan
, Zawawi, Norhasnida
in
Abnormalities
/ Acute toxicity
/ Animals
/ Bacillus amyloliquefaciens
/ Bacteria
/ Bees
/ Biochemical tests
/ Biochemistry
/ Biocompatibility
/ Blood
/ Body Weight
/ Dosage
/ Female
/ functional foods
/ Glycerol
/ Hematology
/ Hemoglobin
/ Honey
/ In vivo methods and tests
/ Oral administration
/ Organs
/ probiotic safety
/ Probiotics
/ Rats
/ Rats, Sprague-Dawley
/ Sprague Dawley rat
/ Subacute toxicity
2023
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![In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey](https://www.mbrl.ae/o/mbrl-theme/images/site-assets/generic/no-book-image.png)
In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey
by
Chan, Kim Wei
, Shafiq Cheng, Mohamad Zulhafiz
, Mohd Lila, Mohd Azmi
, Nur-Fazila, Saulol Hamid
, Khalifa, Shaden A M
, El-Seedi, Hesham R
, Zulkhairi Amin, Fatin Aina
, Ismail, Norsharina
, Sabri, Suriana
, Mohd Esa, Norhaizan
, Zawawi, Norhasnida
in
Abnormalities
/ Acute toxicity
/ Animals
/ Bacillus amyloliquefaciens
/ Bacteria
/ Bees
/ Biochemical tests
/ Biochemistry
/ Biocompatibility
/ Blood
/ Body Weight
/ Dosage
/ Female
/ functional foods
/ Glycerol
/ Hematology
/ Hemoglobin
/ Honey
/ In vivo methods and tests
/ Oral administration
/ Organs
/ probiotic safety
/ Probiotics
/ Rats
/ Rats, Sprague-Dawley
/ Sprague Dawley rat
/ Subacute toxicity
2023
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In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey
![In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey](https://syndetics.com/index.aspx?isbn=/mc.gif&issn=2072-6643&client=MBRL&type=mbrl)
Journal Article
In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee ( Heterotrigona itama) Honey
2023
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Overview
This study evaluated the acute and sub-acute toxicity of
HTI-19 (isolated from stingless bee honey) in female Sprague Dawley rats. In an acute toxicity study, the rats received a low dosage (1 × 10
CFU·mL
), medium dosage (3 × 10
CFU·mL
), or high dosage (1 × 10
CFU·mL
) of
HTI-19 daily orally by syringe-feeding for 14 days. For the subacute toxicity study, rats received a low dosage (1 × 10
CFU·mL
) or a high dosage (1 × 10
CFU·mL
) for 28 days. The probiotic feeding in acute and sub-acute toxicity studies showed no mortality or significant abnormalities in rats throughout the experimental period. In week 2 of the acute study, the body weight of the rats showed a significant increase (
< 0.05) compared to the control. By gross and microscopic examination of organs, no evidently significant changes were observed in the morphology of organs. Serum biochemical tests and blood hematology tests also revealed no treatment-related changes. Overall, these data indicated that oral administration of
HTI-19 up to 1 × 10
CFU·mL
for 28 days can be considered safe.
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