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Mycoplasma agalactiae MAG_5040 is a Mg2+-Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection
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Mycoplasma agalactiae MAG_5040 is a Mg2+-Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection
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Mycoplasma agalactiae MAG_5040 is a Mg2+-Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection
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Journal Article
Mycoplasma agalactiae MAG_5040 is a Mg2+-Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection
2013
Overview
In this study the enzymatic activity of Mycoplasma agalactiae MAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed in Escherichia coli as a GST fusion protein. Recombinant GST-MAG_5040 exhibits nuclease activity similar to typical sugar-nonspecific endo- and exonucleases, with DNA as the preferred substrate and optimal activity in the presence of 20 mM MgCl2 at temperatures ranging from 37 to 45°C. According to in silico analyses, the position of the gene encoding MAG_5040 is consistently located upstream an ABC transporter, in most sequenced mycoplasmas belonging to the Mycoplasma hominis group. In M. agalactiae, MAG_5040 is transcribed in a polycistronic RNA together with the ABC transporter components and with MAG_5030, which is predicted to be a sugar solute binding protein by 3D modeling and homology search. In a natural model of sheep and goats infection, anti-MAG_5040 antibodies were detected up to 9 months post infection. Taking into account its enzymatic activity, MAG_5040 could play a key role in Mycoplasma agalactiae survival into the host, contributing to host pathogenicity. The identification of MAG_5040 opens new perspectives for the development of suitable tools for the control of contagious agalactia in small ruminants.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Animals
/ Antigens, Bacterial - immunology
/ Cloning
/ DNA
/ E coli
/ Gene Expression Regulation, Bacterial
/ Goats
/ Homology
/ Kinases
/ Micrococcal Nuclease - chemistry
/ Micrococcal Nuclease - genetics
/ Micrococcal Nuclease - isolation & purification
/ Micrococcal Nuclease - metabolism
/ Mycoplasma agalactiae - genetics
/ Mycoplasma agalactiae - immunology
/ Mycoplasma agalactiae - metabolism
/ Mycoplasma agalactiae - physiology
/ Mycoplasma Infections - immunology
/ Myelin-associated glycoprotein
/ Nuclease
/ Proteins
/ RNA
/ Sequence Homology, Amino Acid
/ Sheep
/ Sugar
