Journal Article

Two new species of the genus Streptomyces: Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. isolated from the cuticle of Camponotus japonicus Mayr

Xiang, Wensheng, Li, Yao, Wang, Xiangjing, Liu, Chongxi, Jin, Liying, Zheng, Weiwei, Piao, Chenyu, Song, Wei, Yan, Kai

2017

Overview

Two novel actinomycetes, designated strains 2C-SSA16(2) T and 1C-GS8 T , were isolated from the cuticle of Camponotus japonicus Mayr, collected from Northeast Agricultural University, Heilongjiang Province, north China. Both of them contained genes (involved in antibiotics biosynthesis) of the ketosynthase (KS) and methyl malonyl transferase domains (PKS-I) and the adenylation domain (NRPS). A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates 2C-SSA16(2) T and 1C-GS8 T exhibited 98.8% similarity with each other and that they are most closely related to Streptomyces umbrinus JCM 4521 T (99.0, 98.6%), Streptomyces ederensis JCM 4958 T (98.9, 98.7%), Streptomyces aurantiacus JCM 4453 T (98.6, 98.2%), Streptomyces glomeroaurantiacus JCM 4677 T (98.6, 98.1%), Streptomyces tauricus JCM4837 T (98.2, 98.0%) and Streptomyces phaeochromogenes JCM 4070 T (98.2, 99.2%). The corresponding phylogenetic analysis based on partial gyr B gene sequences showed that strains 2C-SSA16(2) T and 1C-GS8 T formed a cluster with the above-mentioned strains. The DNA–DNA hybridization data and phenotypic characteristics indicated that strains 2C-SSA16(2) T and 1C-GS8 T could be readily distinguished from each other and their closest phylogenetic relatives. Therefore, these two strains are suggested to represent two novel species of the genus Streptomyces , for which the names Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. are proposed. The type strains are 2C-SSA16(2) T (=CGMCC 4.7276 T  = DSM 100522 T ) and 1C-GS8 T (=CGMCC 4.7348 = DSM 103127 T ), respectively.

• Publisher: Springer Berlin Heidelberg,Springer Nature B.V
• Subject: Actinomycetes / Adenylation / agricultural colleges / Animals / Antibiotics / Ants - microbiology / Bacterial Typing Techniques / Biochemistry / Biomedical and Life Sciences / Biosynthesis / Biotechnology / Camponotus / Cell Biology / China / Cuticles / Deoxyribonucleic acid / DNA / DNA Gyrase - genetics / DNA topoisomerase / DNA, Bacterial - genetics / DNA, Ribosomal - genetics / Ecology / Fatty Acids - analysis / Gene sequencing / genes / Hybridization / Life Sciences / Microbial Ecology / Microbiology / New species / Nucleic Acid Hybridization / nucleotide sequences / Original Paper / phenotype / Phylogenetics / Phylogeny / ribosomal RNA / RNA, Ribosomal, 16S - genetics / rRNA 16S / sequence analysis / Sequence Analysis, DNA / strains / Streptomyces - classification / Streptomyces - genetics / Streptomyces - isolation & purification / Streptomyces aurantiacus / Streptomyces ederensis / Streptomyces glomeroaurantiacus / Streptomyces phaeochromogenes / Streptomyces tauricus / Streptomyces umbrinus