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Novel mutations in quinolone resistance-determining regions of gyrA, gyrB, parC and parE in Shigella flexneri clinical isolates from eastern Chinese populations between 2001 and 2011
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Novel mutations in quinolone resistance-determining regions of gyrA, gyrB, parC and parE in Shigella flexneri clinical isolates from eastern Chinese populations between 2001 and 2011
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Novel mutations in quinolone resistance-determining regions of gyrA, gyrB, parC and parE in Shigella flexneri clinical isolates from eastern Chinese populations between 2001 and 2011
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Journal Article
Novel mutations in quinolone resistance-determining regions of gyrA, gyrB, parC and parE in Shigella flexneri clinical isolates from eastern Chinese populations between 2001 and 2011
2016
Overview
The aim of this study was to evaluate the prevalence of fluoroquinolone resistance and mechanisms of selected fluoroquinolone resistance in
Shigella flexneri
isolates. A total of 624
S. flexneri
strains isolated between 2001 and 2011 in Jiangsu Province of China were analysed for their fluoroquinolone susceptibility. The quinolone resistance-determining region of
gyrA
,
gyrB
,
parC
and
parE
were amplified and sequenced. In general, 90.5 % of
S. flexneri
exhibited resistance to nalidixic acid. The mean norfloxacin resistance rate was 22.4 % during the 11 years from 2001 to 2011 (6.4 % from 2001 to 2005 and 36.8 % from 2006 to 2011). Sequencing of
gyrA
,
gyrB
,
parC
and
parE
genes of all
S. flexneri
isolates showed that the mutation rate was as high as 93.9 %. In addition, 91.8 % and 92.3 % of
S. flexneri
harboured mutations in
gyrA
and
parC
, respectively. About 35.2 % of
S. flexneri
isolates susceptible to nalidixic acid contained mutations. Meanwhile, mutations were detected in 91.2 % of norfloxacin-susceptible strains, and almost all
S. flexneri
isolates resistant to fluoroquinolone contained mutations. To the best of our knowledge, this is the first study reporting the occurrence of point mutations Asn57Lys and His80Pro in
gyrA
and Ala85Thr, Asp111His and Ser129Pro in
parC
. Emerging fluoroquinolone resistance with a significantly high mutation rate of the
gyrA
and
parC
genes in
S. flexneri
in Jiangsu Province deserves attention, and monitoring antibiotic susceptibility is important for the effective management of
S. flexneri
infections.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
/ Anti-Bacterial Agents - pharmacology
/ Biomedical and Life Sciences
/ Disk Diffusion Antimicrobial Tests
/ DNA Topoisomerases - genetics
/ Dysentery, Bacillary - epidemiology
/ Dysentery, Bacillary - microbiology
/ Humans
/ LDCs
/ Mutation
/ Shigella flexneri - drug effects
/ Shigella flexneri - enzymology
