Journal Article

NcPTP2, a polar tube protein, interacts with spore wall protein in the parasitic microsporidian Nosema ceranae

Ma, Qiang, Dang, Xiaoqun, Xu, Jinshan, Xiong, Liang, Chen, Sheng, Zhou, Zeyang, Wang, Jinglin, Wang, Pengfei, Ma, Zhengang

2024

Overview

Background Microsporidia is an obligate intracellular eukaryote, which is capable of parasitizing vertebrates and invertebrates . Nosema ceranae, which can infect both Apis mellifera and Apis cerana, poses a serious threat and causes heavy losses to the worldwide apiculture. During infection, polar tube, a highly specialized invasion structure, ejected from the spore to deliver the sporoplasm into host cells to cause infection. Although seven different polar tube proteins (PTP1 ~ 7) have been reported from various microsporidia and showed key functions associated with spore invasion and proliferation, no systematic analysis on identification and characterization of polar tube proteins from N. ceranae was found. Methods and results The polar tube proteins 2 (NcPTP2) was identified from the total polar tube proteins of N. ceranae by LC_MS/MS and the transcriptional profile was performed by RT-PCR. Sequence characterization analysis revealed that NcPTP2 was rich in lysine and had a signal peptide at the N-terminal. It had 3 potential O-glycosylation sites and 6 potential N-glycosylation sites. 25 phosphorylation sites were found on serine, tyrosine and threonine sites. Sequence alignment analysis revealed that NcPTP2 was homologous and had conserved cysteine residues with PTP2 proteins from other microsporidia. Indirect immunofuorescence analysis (IFA) and Immunoelectron Microscopy analysis (IEM) confirmed that NcPTP2 was localized on the polar tube of the germinated spores. The interaction between NcPTP2 and spore wall protein in N. ceranae indicated its potential function in anchoring and coiling of polar tube in spore. Conclusion NcPTP2 was the first subcellular localized polar tube protein in N. ceranae and this work could provide an important basis for further analyzing the biological functions of polar tube proteins and uncovering the infection mechanism of N. ceranae to the host cells.

• Publisher: Springer Netherlands,Springer Nature B.V
• Subject: Amino Acid Sequence / Animal Anatomy / Animal Biochemistry / Animals / Apiculture / Apis cerana / Apis mellifera / Bees - microbiology / Biomedical and Life Sciences / Cell proliferation / Cell Wall - metabolism / Conserved sequence / cysteine / eukaryotic cells / Fungal Proteins - genetics / Fungal Proteins - metabolism / Glycosylation / Histology / Immunoelectron microscopy / Infections / Life Sciences / lysine / microscopy / Microsporidia / Morphology / Nosema - genetics / Nosema - metabolism / Nosema ceranae / Nucleotide sequence / Original Article / Phosphorylation / Proteins / sequence alignment / serine / signal peptide / spores / Spores, Fungal - metabolism / Tandem Mass Spectrometry - methods / threonine / transcription (genetics) / Tyrosine