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Distance-based paper analytical devices integrated with molecular imprinted polymers for Escherichia coli quantification
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Journal Article
Distance-based paper analytical devices integrated with molecular imprinted polymers for Escherichia coli quantification
2024
Overview
The development of distance-based paper analytical devices (dPADs) integrated with molecularly imprinted polymers (MIPs) to monitor
Escherichia coli
(
E. coli
) levels in food samples is presented. The fluidic workflow on the device is controlled using a designed hydrophilic bridge valve. Dopamine serves as a monomer for the formation of the
E. coli
–selective MIP layer on the dPADs. The detection principle relies on the inhibition of the
E. coli
toward copper (II) (Cu
2+
)-triggered oxidation of o-phenylenediamine (OPD) on the paper substrate. Quantitative detection is simply determined through visual observation of the residual yellow color of the OPD in the detection zone, which is proportional to
E. coli
concentration. The sensing exhibits a linear range from 25.0 to 1200.0 CFU mL
−1
(
R
2
= 0.9992) and a detection limit (LOD) of 25.0 CFU mL
−1
for
E. coli
detection. Additionally, the technique is highly selective with no interference even from the molecules that have shown to react with OPD to form oxidized OPD. The developed device demonstrates accuracy and precision for
E. coli
quantification in food samples with recovery percentages between 98.3 and 104.7% and the highest relative standard deviation (RSD) of 4.55%.
T
-test validation shows no significant difference in
E. coli
concentration measured between our method and a commercial assay. The proposed dPAD sensor has the potential for selective and affordable
E. coli
determination in food samples without requiring sample preparation. Furthermore, this strategy can be extended to monitor other molecules for which MIP can be developed and integrated into paper-microfluidic platform.
Graphical Abstract
Publisher
Springer Vienna,Springer,Springer Nature B.V
Subject
