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The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling
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The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling
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The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling
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Journal Article
The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling
2018
Overview
The leucine-rich repeat receptor kinase (LRR-RK) BRASSINOSTEROID INSENSITIVE 1 (BRI1) requires a shape-complementary SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) co-receptor for brassinosteroid sensing and receptor activation
1
. Interface mutations that weaken the interaction between receptor and co-receptor in vitro reduce brassinosteroid signalling responses
2
. The SERK3
elongated
(
elg
) allele
3
–
5
maps to the complex interface and shows enhanced brassinosteroid signalling, but surprisingly no tighter binding to the BRI1 ectodomain in vitro. Here, we report that rather than promoting the interaction with BRI1, the
elg
mutation disrupts the ability of the co-receptor to interact with the ectodomains of BRI1-ASSOCIATED-KINASE1 INTERACTING KINASE (BIR) receptor pseudokinases, negative regulators of LRR-RK signalling
6
. A conserved lateral surface patch in BIR LRR domains is required for targeting SERK co-receptors and the
elg
allele maps to the core of the complex interface in a 1.25 Å BIR3–SERK1 structure. Collectively, our structural, quantitative biochemical and genetic analyses suggest that brassinosteroid signalling complex formation is negatively regulated by BIR receptor ectodomains.
Receptor BRI1 interacts with SERK co-receptors to initiate brassinosteroid signalling. BIR pseudokinases are negative regulators. Here, structural and biological data show that BIRs bind SERKs through a lateral interaction site.
