Journal Article

Anti‐arthritic effect of chicken embryo tissue hydrolyzate against adjuvant arthritis in rats (X‐ray microtomographic and histopathological analysis)

Rzhepakovsky, Igor, Heinz, Volker, Timchenko, Lyudmila, Fritsch, Tilman, Vukovic, Marko, Anusha Siddiqui, Shahida, Nagdalian, Andrey, Piskov, Sergey, Kochergin, Stanislav, Dolgalev, Alexander, Sizonenko, Marina, Benlidayi, Mehmet, Avanesyan, Svetlana, Enukashvily, Natella, Grimm, Wolf‐Dieter, Dhingra, Kunaal

2021

Overview

Finding new, safe strategies to prevent and control rheumatoid arthritis is an urgent task. Bioactive peptides and peptide‐rich protein hydrolyzate represent a new trend in the development of functional foods and nutraceuticals. The resulting tissue hydrolyzate of the chicken embryo (CETH) has been evaluated for acute toxicity and tested against chronic arthritis induced by Freund's full adjuvant (modified Mycobacterium butyricum) in rats. The antiarthritic effect of CETH was studied on the 28th day of the experiment after 2 weeks of oral administration of CETH at doses of 60 and 120 mg/kg body weight. Arthritis was evaluated on the last day of the experiment on the injected animal paw using X‐ray computerized microtomography and histopathology analysis methods. The CETH effect was compared with the non‐steroidal anti‐inflammatory drug diclofenac sodium (5 mg/kg). Oral administration of CETH was accompanied by effective dose‐dependent correction of morphological changes caused by the adjuvant injection. CETH had relatively high recovery effects in terms of parameters for reducing inflammation, inhibition of osteolysis, reduction in the inflammatory reaction of periarticular tissues, and cartilage degeneration. This study presents for the first time that CETH may be a powerful potential nutraceutical agent or bioactive component in the treatment of rheumatoid arthritis. Finding new, safe strategies to prevent and control rheumatoid arthritis is an urgent task. Bioactive peptides and peptide‐rich protein hydrolyzate represent a new trend in the development of functional foods and nutraceuticals. This study presents for the first time that CETH may be a powerful potential nutraceutical agent or bioactive component in the treatment of rheumatoid arthritis.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc
• Subject: Acute toxicity / adjuvant arthritis / Amino acids / Animal tissues / antiarthritic effect / Antioxidants / Arthritis / Biocompatibility / Biological activity / Biological products / Biomedical materials / Body weight / Cartilage / chicken embryo tissue / Chickens / Degeneration / Diclofenac / Disease / Drugs / Eggs / Embryos / food‐derived bioactive peptides / Functional foods & nutraceuticals / histopathological analysis / Histopathology / hydrolyzate / in vitro and in vivo assays / Inflammation / Laboratory animals / Microtomography / Oral administration / Original Research / Osteolysis / Peptides / Proteins / Rheumatoid arthritis / Steroids / Toxicity testing / X‐ray microtomography