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Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates
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Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates
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Journal Article
Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates
2010
Overview
We have characterized an assay measuring CD8 T cell-mediated inhibition of human immunodeficiency virus (HIV) type 1 replication, demonstrating specificity and reproducibility and employing a panel of primary HIV-1 isolates. The assay uses relatively simple autologous cell culture and enzyme-linked immunosorbent assay, avoids generation of T cell clones, and can be performed with <2 million peripheral blood mononuclear cells. Efficient CD8 T cell-mediated cross-clade inhibition of HIV-1 replication in vitro was demonstrated in antiretroviral therapy-naive HIV-1-infected subjects with controlled viral replication in vivo but not in viremic subjects. An HIV-1 vaccine candidate, consisting of DNA and recombinant adenovirus 5 vectors tested in a phase I clinical trial, induced CD8 T cells that efficiently inhibited HIV-1 in a HLA-I-dependent manner. Assessment of direct antiviral T cell function by this assay provides additional information to guide vaccine design and the prioritizing of candidates for further clinical trials.
Publisher
The University of Chicago Press,University of Chicago Press,Oxford University Press
Subject
/ Adenoviruses, Human - genetics
/ Adult
/ Aged
/ Biological and medical sciences
/ CD8-Positive T-Lymphocytes - immunology
/ Enzyme linked immunospot assay
/ Enzyme-Linked Immunosorbent Assay - methods
/ Female
/ Fundamental and applied biological sciences. Psychology
/ HIV
/ HIV 1
/ HIV/AIDS
/ Human immunodeficiency virus 1
/ Humans
/ Male
/ Neutralization Tests - methods
/ Placebos
/ Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
/ Virology
/ Virus Replication - immunology
/ Viruses
