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new chiral derivatizing agent for the HPLC separation of α-amino acids on a standard reverse-phase column
by
Kotthaus, A. F
, Altenbach, H.-J
in
Absorption
/ acids
/ Adsorption
/ Amino acids
/ Amino Acids - chemistry
/ Amino Acids - isolation & purification
/ Analytical Chemistry
/ Biochemical Engineering
/ Biochemistry
/ Biomedical and Life Sciences
/ Chiral derivatization agent (CDA)
/ Chromatography, High Pressure Liquid - instrumentation
/ Chromatography, High Pressure Liquid - methods
/ Diastereoisomers
/ diastereomers
/ Enantiomeric purity determination
/ High-performance liquid chromatography
/ Life Sciences
/ Marfey reagent
/ Neurobiology
/ Original Article
/ Proteomics
/ Reagents
/ Resins, Synthetic - chemistry
/ reversed-phase high performance liquid chromatography
/ Sanger reagent
/ wavelengths
2011
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new chiral derivatizing agent for the HPLC separation of α-amino acids on a standard reverse-phase column
by
Kotthaus, A. F
, Altenbach, H.-J
in
Absorption
/ acids
/ Adsorption
/ Amino acids
/ Amino Acids - chemistry
/ Amino Acids - isolation & purification
/ Analytical Chemistry
/ Biochemical Engineering
/ Biochemistry
/ Biomedical and Life Sciences
/ Chiral derivatization agent (CDA)
/ Chromatography, High Pressure Liquid - instrumentation
/ Chromatography, High Pressure Liquid - methods
/ Diastereoisomers
/ diastereomers
/ Enantiomeric purity determination
/ High-performance liquid chromatography
/ Life Sciences
/ Marfey reagent
/ Neurobiology
/ Original Article
/ Proteomics
/ Reagents
/ Resins, Synthetic - chemistry
/ reversed-phase high performance liquid chromatography
/ Sanger reagent
/ wavelengths
2011
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new chiral derivatizing agent for the HPLC separation of α-amino acids on a standard reverse-phase column
by
Kotthaus, A. F
, Altenbach, H.-J
in
Absorption
/ acids
/ Adsorption
/ Amino acids
/ Amino Acids - chemistry
/ Amino Acids - isolation & purification
/ Analytical Chemistry
/ Biochemical Engineering
/ Biochemistry
/ Biomedical and Life Sciences
/ Chiral derivatization agent (CDA)
/ Chromatography, High Pressure Liquid - instrumentation
/ Chromatography, High Pressure Liquid - methods
/ Diastereoisomers
/ diastereomers
/ Enantiomeric purity determination
/ High-performance liquid chromatography
/ Life Sciences
/ Marfey reagent
/ Neurobiology
/ Original Article
/ Proteomics
/ Reagents
/ Resins, Synthetic - chemistry
/ reversed-phase high performance liquid chromatography
/ Sanger reagent
/ wavelengths
2011
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new chiral derivatizing agent for the HPLC separation of α-amino acids on a standard reverse-phase column
Journal Article
new chiral derivatizing agent for the HPLC separation of α-amino acids on a standard reverse-phase column
2011
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Overview
A new chiral derivatizing agent for α-amino acids is described which leads to diastereomers that can be separated by reverse-phase HPLC with direct detection by a diode array detector. The main advantage of the presented procedure is the fact that an excess of the derivatizing reagent can be employed as the product exhibits an absorption maximum at 360 nm, while the reagent has its absorption maximum at 260 nm. Therefore, it is possible to suppress the reagent signal by a detection wavelength of 400 nm leading to an easy and general method for the enantioseparation of a mixture of dl-amino acids and the determination of the enantiomeric purity of α-amino acid as exemplified by 16 different α-amino acids.
Publisher
Vienna : Springer Vienna,Springer Vienna,Springer Nature B.V
Subject
/ acids
/ Amino Acids - isolation & purification
/ Biomedical and Life Sciences
/ Chiral derivatization agent (CDA)
/ Chromatography, High Pressure Liquid - instrumentation
/ Chromatography, High Pressure Liquid - methods
/ Enantiomeric purity determination
/ High-performance liquid chromatography
/ Reagents
/ Resins, Synthetic - chemistry
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