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Expression of Periplasmic Chaperones in Salmonella Typhimurium Reduces Its Viability In Vivo
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Expression of Periplasmic Chaperones in Salmonella Typhimurium Reduces Its Viability In Vivo
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Journal Article
Expression of Periplasmic Chaperones in Salmonella Typhimurium Reduces Its Viability In Vivo
2015
Overview
The efficacy of live attenuated bacterial vectors is dependent upon the fine-tuning of a strain’s immunogenicity and its virulence. Strains are often engineered to deliver heterologous antigens, but soluble expression of recombinant proteins can be troublesome. Therefore, secretion systems or chaperone proteins are routinely used to assist in attaining high levels of functional, soluble protein production. However, the effects of chaperone expression on the virulence of attenuated bacterial vectors have not been previously reported. In anticipation of utilizing periplasmic chaperone proteins to facilitate soluble production of immunomodulatory proteins in an attenuated strain of
Salmonella
Typhimurium, the production of the chaperones was tested for their effect on both culture growth and bacterial persistence in mouse tissues. Although no effect on growth of the bacteria was observed in vitro, the increased expression of the periplasmic chaperones resulted in over-attenuation of the
Salmonella
in vivo.
Publisher
Springer US,Springer Nature B.V
Subject
/ Antigens
/ Bacteria
/ Biomedical and Life Sciences
/ Mice
/ Microbial Viability - genetics
/ Molecular Chaperones - genetics
/ Molecular Chaperones - metabolism
/ Proteins
/ Salmonella Infections - microbiology
/ Salmonella typhimurium - genetics
/ Salmonella typhimurium - growth & development
