Journal Article

Evaluation of serum MicroRNA 21, MicroRNA 192 and serum TGFβ1 in type 2 diabetes mellitus patients and their relation to diabetic nephropathy

Kamel, Laila Nessim, Waked, Emam Abdalatif, Elhanafi, Hadeel Mohammad, Shawky Amin, Hanan, Abou Eleila, Jumana Gamal, Abdel Wahab Mohamed, Amal

2024

Overview

BackgroundDiabetic nephropathy (DN) is a frequent and long-lasting microvascular consequence that has an established connection with diabetes. It serves as the primary etiological agent of end-stage renal disease, a critical renal disorder that develops on a worldwide level. The molecular pathophysiology of DN is multifactorial, such as transforming growth factor-beta [TGF-β] which affects the expression of miRNAs such as miRNA-21 and miRNA-192 during renal fibrosis. However, to date, the clinical application is inadequate due to discrepancies observed in the published data. This cross-sectional investigation aimed to assess the correlation between serum TGF-β1, miRNA-21 and 192, and glycemic control, metabolic abnormalities, and renal function in patients with type II diabetes.MethodsBased on the albumin/creatinine ratio (ACR), fifty subjects with type II diabetes were divided into three categories: Group I consisted of individuals with normoalbuminuria (n = 16), Group II of microalbuminuria (n = 16), and Group III of overt proteinuria (n = 18). All participants were subjected to the estimation of mature miRNA-21 and miRNA-192 by TaqMan two-step stem loop qRT-PCR and serum TGFβ1 level by ELISA.ResultsThere was an upregulation in miRNA-21 expression in the 3 different groups of patients (p value = 0.043). The serum fold change (FC) of miRNA-21 showed significantly greater median values in patients with overt proteinuria compared to those with normoalbuminuria (5.57 FC versus 1.11 FC, p = 0.017). A positive correlation (r = 0.343) (p = 0.013) was observed between the ACR and the median levels of miRNA-21, which was statistically significant. No statistically significant distinctions were detected in the concentrations of serum TGF-β1 or miRNA-192 among the three patient groups (p values of 0.234 and 0.225, respectively).ConclusionThe findings of the present research implied that miRNA-21 might function as an early indicator of renal pathology associated with diabetes mellitus (DM).

• Publisher: Springer Nature B.V
• Subject: Age / Autoimmune diseases / Creatinine / Diabetes / Diabetes mellitus (non-insulin dependent) / Diabetic nephropathy / End-stage renal disease / Extracellular matrix / Females / Fibrosis / Genes / Glucose / Hemoglobin / Hypertension / Inflammation / Kidney diseases / Kinases / Metabolism / MicroRNAs / Microvasculature / miRNA / Nephropathy / Pathogenesis / Patients / Plasma / Proteinuria / Renal function / Statistical analysis / Transforming growth factor-b / Transforming growth factor-b1