Journal Article

IFN-γ induces aberrant CD49b+ NK cell recruitment through regulating CX3CL1: a novel mechanism by which IFN-γ provokes pregnancy failure

Liu, H-Y, Song, Z-H, Li, Z-Y, Li, L-L, Chao, H-H, Yang, Y, Zhang, Y-J, Peng, J-P

2014

Overview

Interferon- γ (IFN- γ ), a pleiotropic lymphokine, has important regulatory effects on many cell types. Although IFN- γ is essential for the initiation of uterine vascular modifications and maintenance of decidual integrity, IFN- γ administration can also cause pregnancy failure in many species. However, little is known about the effector mechanisms involved. In this study, using an IFN- γ -induced abortion mouse model, we reported that no Dolichos biflorus agglutinin lectin-positive uterine natural killer (uNK) cells were observed in the uteri from IFN- γ -induced abortion mice. By contrast, the percentage of CD3 − CD49b + NK cells in the uterus and blood from a foetal resorption group was significantly higher than that of the control group. Similarly, significantly upregulated expression of CD49b (a pan-NK cell marker), CX3CL1 and CX3CR1 (CX3CL1 receptor) was detected in the uteri of IFN- γ -induced abortion mice. Using isolated uterine stromal cells, we showed that upregulated expression of CX3CL1 by IFN- γ was dependent on a Janus family kinase 2-signal transducers and activators of transcription 1 (JAK2-STAT1) pathway. We further demonstrated the chemotactic activity of CX3CL1 in uterine stromal cell conditioned medium on primary splenic NK cells. Finally, we observed increased recruitment of CD49b + NK cells into the endometrium after exogenous CX3CL1 administration. Collectively, our findings indicate that IFN- γ can significantly increase uterine CX3CL1 expression via activation of the JAK2-STAT1 pathway, thus inducing CD49b + NK cell uterine homing, and eventually provoke foetal loss. Thus, we provide a new line of evidence correlating the deleterious effects of IFN- γ on pregnancy with the aberrant regulation of CX3CL1 and CD49b + NK cells.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13/1 / 13/31 / 13/51 / 14/19 / 38/77 / 631/250/1619/382 / 631/80/86 / 64/60 / 692/420/256 / 96/95 / Abortion, Induced / Animals / Antibodies / Biochemistry / Biomedical and Life Sciences / Cell Biology / Cell Culture / Cells, Cultured / Chemokine CX3CL1 - genetics / Chemokine CX3CL1 - metabolism / Chemokine CX3CL1 - pharmacology / Chemotaxis - drug effects / CX3C Chemokine Receptor 1 / Female / Fetus / Gene Expression Regulation / Immunology / Integrin alpha2 - genetics / Integrin alpha2 - metabolism / Interferon-gamma - genetics / Interferon-gamma - metabolism / Interferon-gamma - pharmacology / Janus Kinase 2 - genetics / Janus Kinase 2 - metabolism / Killer Cells, Natural - cytology / Killer Cells, Natural - drug effects / Killer Cells, Natural - metabolism / Life Sciences / Mice / Mice, Inbred BALB C / Original / original-article / Pregnancy / Receptors, Chemokine - genetics / Receptors, Chemokine - metabolism / Receptors, Mitogen - genetics / Receptors, Mitogen - metabolism / Signal Transduction / STAT1 Transcription Factor - genetics / STAT1 Transcription Factor - metabolism / Stromal Cells - cytology / Stromal Cells - drug effects / Stromal Cells - metabolism / Uterus - cytology / Uterus - drug effects / Uterus - metabolism