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Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis
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Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis
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Journal Article
Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis
2012
Overview
Plants respond to various environmental stresses by activating “stress genes”.
CIPKs
(CBL-interacting protein kinases) family genes play an important role in the process of stress response. In this study, a CIPK gene
MdSOS2
was isolated from apple (
Malus
×
Domestica
). Sequence alignment and phylogenetic analysis showed that it is highly similar with
Arabidopsis
AtSOS2
and contained the conserved domains and motifs. Expression analysis demonstrated that
MdSOS2
expressed in all tested organs at different levels, and positively in response to salt stress. Furthermore, the ectopic expression of
MdSOS2
complemented the function of Arabidopsis
sos2
mutant, and conferred enhanced salt tolerance to the transgenic
Arabidopsis
. Yeast two-hybrid assay indicated that the N-terminal of MdSOS2 protein physically interacted with MdSOS3 and AtSOS3, respectively, suggesting that SOS pathway operates in apple tree. Finally,
MdSOS2
overexpression enhanced, while its suppression reduced the tolerance to salt in transgenic apple calluses, indicating that
MdSOS2
acts as a positive regulator in response to salt stress in apple.
Publisher
Springer-Verlag,Springer,Springer Nature B.V
Subject
/ Apples
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ callus
/ Cloning
/ Fruits
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Plant
/ Gene Expression Regulation, Plant - genetics
/ genes
/ Genetic Complementation Test
/ genetics
/ Malus
/ mutants
/ Mutation
/ Plants, Genetically Modified
/ Protein Serine-Threonine Kinases
/ Protein-Serine-Threonine Kinases - genetics
/ Protein-Serine-Threonine Kinases - metabolism
/ proteins
/ Signal Transduction - genetics
/ Stress, Physiological - genetics
/ trees
/ Two-Hybrid System Techniques
/ Yeasts
