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Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis
by
Sahi, Shivendra V.
, Shukla, Devesh
, Kiiskila, Jeffrey
, Datta, Rupali
, Jain, Ajay
, Sharma, Nilesh
, Krishnamurthy, Sneha
, Tiwari, Manish
in
14/63
/ 38
/ 38/39
/ 38/61
/ 49
/ 631/449
/ Arabidopsis - genetics
/ Arabidopsis - metabolism
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Basic Helix-Loop-Helix Transcription Factors - genetics
/ Basic Helix-Loop-Helix Transcription Factors - metabolism
/ Biosynthesis
/ Biotransformation
/ Chlorides - chemistry
/ Chlorides - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Fabrication
/ Gene Expression Profiling
/ Gene Expression Regulation, Plant
/ Gene families
/ Glutathione
/ Glutathione Transferase - genetics
/ Glutathione Transferase - metabolism
/ Gold
/ Gold - chemistry
/ Gold - metabolism
/ Gold Compounds - chemistry
/ Gold Compounds - metabolism
/ Green chemistry
/ Green Chemistry Technology
/ Heavy metals
/ Homeostasis
/ Humanities and Social Sciences
/ Indoleacetic Acids - metabolism
/ Metal Nanoparticles - chemistry
/ multidisciplinary
/ Nanoparticles
/ Nanotechnology
/ Plant hormones
/ Plant Roots - genetics
/ Plant Roots - metabolism
/ Plant Shoots - genetics
/ Plant Shoots - metabolism
/ Protein Isoforms - genetics
/ Protein Isoforms - metabolism
/ Proteome - genetics
/ Proteome - metabolism
/ Proteomes
/ Science
/ Seedlings
/ Seedlings - genetics
/ Seedlings - metabolism
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Transcriptome
2016
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Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis
by
Sahi, Shivendra V.
, Shukla, Devesh
, Kiiskila, Jeffrey
, Datta, Rupali
, Jain, Ajay
, Sharma, Nilesh
, Krishnamurthy, Sneha
, Tiwari, Manish
in
14/63
/ 38
/ 38/39
/ 38/61
/ 49
/ 631/449
/ Arabidopsis - genetics
/ Arabidopsis - metabolism
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Basic Helix-Loop-Helix Transcription Factors - genetics
/ Basic Helix-Loop-Helix Transcription Factors - metabolism
/ Biosynthesis
/ Biotransformation
/ Chlorides - chemistry
/ Chlorides - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Fabrication
/ Gene Expression Profiling
/ Gene Expression Regulation, Plant
/ Gene families
/ Glutathione
/ Glutathione Transferase - genetics
/ Glutathione Transferase - metabolism
/ Gold
/ Gold - chemistry
/ Gold - metabolism
/ Gold Compounds - chemistry
/ Gold Compounds - metabolism
/ Green chemistry
/ Green Chemistry Technology
/ Heavy metals
/ Homeostasis
/ Humanities and Social Sciences
/ Indoleacetic Acids - metabolism
/ Metal Nanoparticles - chemistry
/ multidisciplinary
/ Nanoparticles
/ Nanotechnology
/ Plant hormones
/ Plant Roots - genetics
/ Plant Roots - metabolism
/ Plant Shoots - genetics
/ Plant Shoots - metabolism
/ Protein Isoforms - genetics
/ Protein Isoforms - metabolism
/ Proteome - genetics
/ Proteome - metabolism
/ Proteomes
/ Science
/ Seedlings
/ Seedlings - genetics
/ Seedlings - metabolism
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Transcriptome
2016
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Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis
by
Sahi, Shivendra V.
, Shukla, Devesh
, Kiiskila, Jeffrey
, Datta, Rupali
, Jain, Ajay
, Sharma, Nilesh
, Krishnamurthy, Sneha
, Tiwari, Manish
in
14/63
/ 38
/ 38/39
/ 38/61
/ 49
/ 631/449
/ Arabidopsis - genetics
/ Arabidopsis - metabolism
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Basic Helix-Loop-Helix Transcription Factors - genetics
/ Basic Helix-Loop-Helix Transcription Factors - metabolism
/ Biosynthesis
/ Biotransformation
/ Chlorides - chemistry
/ Chlorides - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Fabrication
/ Gene Expression Profiling
/ Gene Expression Regulation, Plant
/ Gene families
/ Glutathione
/ Glutathione Transferase - genetics
/ Glutathione Transferase - metabolism
/ Gold
/ Gold - chemistry
/ Gold - metabolism
/ Gold Compounds - chemistry
/ Gold Compounds - metabolism
/ Green chemistry
/ Green Chemistry Technology
/ Heavy metals
/ Homeostasis
/ Humanities and Social Sciences
/ Indoleacetic Acids - metabolism
/ Metal Nanoparticles - chemistry
/ multidisciplinary
/ Nanoparticles
/ Nanotechnology
/ Plant hormones
/ Plant Roots - genetics
/ Plant Roots - metabolism
/ Plant Shoots - genetics
/ Plant Shoots - metabolism
/ Protein Isoforms - genetics
/ Protein Isoforms - metabolism
/ Proteome - genetics
/ Proteome - metabolism
/ Proteomes
/ Science
/ Seedlings
/ Seedlings - genetics
/ Seedlings - metabolism
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Transcriptome
2016
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Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis
Journal Article
Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis
2016
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Overview
A large number of plants have been tested and exploited in search of a green chemistry approach for the fabrication of gold or other precious metal nanomaterials. Despite the potential of plant based methods, very little is known about the underlying biochemical reactions and genes involved in the biotransformation mechanism of AuCl
4
into gold nanoparticles (AuNPs). In this research, we thus focused on studying the effect of Au on growth and nanoparticles formation by analyses of transcriptome, proteome and ionome shift in
Arabidopsis
. Au exposure favored the growth of
Arabidopsis
seedling and induced formation of nanoparticles in root and shoot, as indicated by optical and hyperspectral imaging. Root transcriptome analysis demonstrated the differential expression of the members of
WRKY
,
MYB
and
BHLH
gene families, which are involved in the Fe and other essential metals homeostasis. The proteome analysis revealed that Glutathione S-transferases were induced in the shoot and suggested its potential role in the biosynthesis AuNPs. This study also demonstrated the role of plant hormone auxin in determining the Au induced root system architecture. This is the first study using an integrated approach to understand the
in planta
biotransformation of KAuCl
4
into AuNPs.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38
/ 38/39
/ 38/61
/ 49
/ 631/449
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Basic Helix-Loop-Helix Transcription Factors - genetics
/ Basic Helix-Loop-Helix Transcription Factors - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Gene Expression Regulation, Plant
/ Glutathione Transferase - genetics
/ Glutathione Transferase - metabolism
/ Gold
/ Humanities and Social Sciences
/ Indoleacetic Acids - metabolism
/ Metal Nanoparticles - chemistry
/ Protein Isoforms - metabolism
/ Science
/ Transcription Factors - genetics
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