Journal Article

Therapeutic potential of red bean (phaseolus vulgaris) peptides: anticancer, antihypertension, and antioxidant activities

Kittisenachai, Suthathip, Charoenlappanit, Sawanya, Jaresitthikunchai, Janthima, Thaisakun, Siriwan, Supaibulwatana, Kanyaratt, Roytrakul, Sittiruk, Phaonakrop, Narumon

2025

Overview

Red beans ( Phaseolus vulgaris ) are gaining recognition for their potential in functional foods and nutraceuticals. This study isolated bioactive peptides under 3 kDa from red bean protein hydrolysate, generated by pepsin digestion and purified using sequential cation exchange, anion exchange, and reverse-phase chromatography. The resulting hydrolysate exhibited anticancer activity. Following chromatographic fractionation and LC-MS sequencing, eight candidate peptides were synthesized and comparatively assessed for bioactivity. Remarkably, peptide RB-8 (LIIPATSTKFL) demonstrated significant anticancer activity against colorectal (CaCO2) and cervical (SiHa) cancer cells through distinct mechanistic pathways. Furthermore, RB-7 (RGSKQRQKRQW) showed potent antihypertensive and DPPH radical scavenging activities, RB-6 (RRLRILL) displayed the strongest ABTS radical scavenging activity, and RB-1 (NLRKLKRL) exhibited the highest FRAP. These findings highlight the potential of red bean-derived peptides as natural agents for managing non-communicable diseases (NCDs) and their applicability in the food industry.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/449 / 631/61 / Amino acids / Anion exchange / Anticancer peptide / Antihypertensive Agents - chemistry / Antihypertensive Agents - pharmacology / Antihypertensive peptide / Antihypertensives / Antineoplastic Agents - chemistry / Antineoplastic Agents - pharmacology / Antioxidant peptide / Antioxidants / Antioxidants - chemistry / Antioxidants - pharmacology / Antitumor activity / Beans / Biological activity / Caco-2 Cells / Cancer / Cardiovascular disease / Cation exchange / Cell Line, Tumor / Chromatography / Diabetes / Experiments / Food industry / Fractionation / Functional foods & nutraceuticals / Humanities and Social Sciences / Humans / Hydrolysates / Hypertension / multidisciplinary / Pepsin / Peptides / Peptides - chemistry / Peptides - isolation & purification / Peptides - pharmacology / Phaseolus - chemistry / Physicochemical properties / Plant Proteins - chemistry / Plant Proteins - pharmacology / Protein hydrolysate / Proteins / Science / Science (multidisciplinary)