Asset Details
Do you wish to reserve the book?
A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes
Do you wish to request the book?
A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes
2025
Overview
Anopheles
mosquitoes are the sole vector of malaria, the most burdensome vector-borne disease worldwide. At present, strategies for reducing mosquito populations or limiting their ability to transmit disease show the most promise for disease control. Therefore, improving our understanding of mosquito biology and immune function may aid new approaches to limit malaria transmission. Here, we perform genome-wide CRISPR screens in
Anopheles
mosquito cells to identify genes required for fitness and that confer resistance to clodronate liposomes, which are used to ablate immune cells. The cellular fitness screen identifies 1280 fitness-related genes (393 at highest confidence) that are highly enriched for roles in fundamental cell processes. The clodronate screen identifies resistance factors that impair clodronate liposome function. For the latter, we confirm roles in liposome uptake and processing through in vivo validation in
Anopheles gambiae
that provide new mechanistic detail of phagolysosome formation and clodronate liposome processing. Altogether, we present a genome-wide CRISPR knockout platform in a major malaria vector and identify genes important for fitness and immune-related processes.
Mosquitoes are major vectors for the transmission of many serious pathogens. This study uses genome-wide CRISPR screens in the mosquito, Anopheles gambiae, to reveal new insights into mosquito fitness and the function of clodronate-liposome mediated immune cell ablation.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 45/47
/ Ablation
/ Animals
/ Cells
/ Clodronic Acid - pharmacology
/ Cloning
/ Clustered Regularly Interspaced Short Palindromic Repeats
/ CRISPR
/ Female
/ Fitness
/ Genes
/ Genomes
/ Humanities and Social Sciences
/ Insects
/ Malaria
/ Malaria - prevention & control
/ Mosquito Vectors - drug effects
/ Mosquito Vectors - immunology
/ Ontology
/ RNA
/ Science
/ Vectors
