Journal Article

Subgingival microbiome in patients with healthy and ailing dental implants

Wang, Zicheng, Xu, Lixin, Li, Lianshuo, Zhang, Jieni, Lin, Jiuxiang, Zheng, Hui, Zhang, Qian, Chen, Ting, Chen, Feng

2015

Overview

Dental implants are commonly used to replace missing teeth. However, the dysbiotic polymicrobial communities of peri-implant sites are responsible for peri-implant diseases, such as peri-implant mucositis and peri-implantitis. In this study, we analyzed the microbial characteristics of oral plaque from peri-implant pockets or sulci of healthy implants (n = 10), peri-implant mucositis (n = 8) and peri-implantitis (n = 6) sites using pyrosequencing of the 16S rRNA gene. An increase in microbial diversity was observed in subgingival sites of ailing implants, compared with healthy implants. Microbial co-occurrence analysis revealed that periodontal pathogens, such as Porphyromonas gingivalis , Tannerella forsythia and Prevotella intermedia , were clustered into modules in the peri-implant mucositis network. Putative pathogens associated with peri-implantitis were present at a moderate relative abundance in peri-implant mucositis, suggesting that peri-implant mucositis an important early transitional phase during the development of peri-implantitis. Furthermore, the relative abundance of Eubacterium was increased at peri-implantitis locations and co-occurrence analysis revealed that Eubacterium minutum was correlated with Prevotella intermedia in peri-implantitis sites, which suggests the association of Eubacterium with peri-implantitis. This study indicates that periodontal pathogens may play important roles in the shifting of healthy implant status to peri-implant disease.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 49/22 / 49/23 / 631/326/2565/855 / 692/420/254 / Abundance / Adult / Bacterial Typing Techniques / Bacteroides - classification / Bacteroides - genetics / Bacteroides - isolation & purification / Case-Control Studies / Dental implants / Dental Implants - microbiology / Dental prosthetics / Dental restorative materials / Eubacterium - classification / Eubacterium - genetics / Eubacterium - isolation & purification / Female / Genes, Bacterial / Humanities and Social Sciences / Humans / Male / Microbiota - genetics / Middle Aged / Mucositis / multidisciplinary / Pathogens / Peri-Implantitis - diagnosis / Peri-Implantitis - microbiology / Peri-Implantitis - pathology / Periodontal Pocket - diagnosis / Periodontal Pocket - microbiology / Periodontal Pocket - pathology / Periodontics / Phylogeny / Porphyromonas gingivalis - classification / Porphyromonas gingivalis - genetics / Porphyromonas gingivalis - isolation & purification / Prevotella intermedia - classification / Prevotella intermedia - genetics / Prevotella intermedia - isolation & purification / Relative abundance / RNA, Ribosomal, 16S - genetics / rRNA 16S / Science / Stomatitis - diagnosis / Stomatitis - microbiology / Stomatitis - pathology / Teeth / Transplants & implants