Asset Details
Do you wish to reserve the book?
Poly(styrene-4-sulfonate)-protected copper nanoclusters as a fluorometric probe for sequential detection of cytochrome c and trypsin
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Poly(styrene-4-sulfonate)-protected copper nanoclusters as a fluorometric probe for sequential detection of cytochrome c and trypsin
Do you wish to request the book?
Poly(styrene-4-sulfonate)-protected copper nanoclusters as a fluorometric probe for sequential detection of cytochrome c and trypsin
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Poly(styrene-4-sulfonate)-protected copper nanoclusters as a fluorometric probe for sequential detection of cytochrome c and trypsin
2018
Overview
Stable copper nanoclusters (CuNCs) were prepared by utilizing D-penicillamine as both the stabilizer and reductant. The emission of the CuNCs (with excitation/emission peaks at 390/645 nm) is largely stabilized by coating with poly(sodium-p-styrenesulfonate) (PSS). Cytochrome c (Cyt c) quenches the fluorescence of the PSS-coated CuNCs, and this effect was exploited to design a quenchometric fluorometric assay for Cyt c. If trypsin is added to the loaded CuNCs, it will hydrolyze Cyt c to form peptide fragments, and fluorescence is gradually restored. A highly sensitive and fluorometric turn-off-on assay was constructed for sequential detection of Cyt c and trypsin. The linear ranges for Cyt c and trypsin are from 8.0 nM to 680 nM, and from 0.1 to 6.0 μg mL
−1
, and the lower detection limits are 0.83 nM and 20 ng mL
−1
for Cyt c and trypsin, respectively.
Graphical abstract
Schematic illustration of the fluorometric assay for trypsin based on the electron transfer between poly(p-styrenesulfonate)-protected copper nanoclusters (PSS-CuNCs) and cytochrome c (Cyt c).
Publisher
Springer Vienna,Springer,Springer Nature B.V
