Journal Article

n-3 Fatty Acid Supplementation and Leukocyte Telomere Length in Patients with Chronic Kidney Disease

Watts, Gerald, O’Callaghan, Nathan, Burke, Valerie, Mori, Trevor, Fenech, Michael, Huang, Rae-Chi, Beilin, Lawrence, Barden, Anne, Puddey, Ian, Mas, Emile, Irish, Ashley

2016

Overview

DNA telomere shortening associates with the age-related increase cardiovascular disease (CVD) risk. Reducing oxidative stress, could modify telomere erosion during cell replication, and CVD risk in patients with chronic kidney disease (CKD). The effect of n-3 fatty acids and coenzyme Q10 (CoQ) on telomere length was studied in a double-blind placebo-controlled trial in CKD. Eighty-five CKD patients were randomized to: n-3 fatty acids (4 g); CoQ (200 mg); both supplements; or control (4 g olive oil), daily for 8 weeks. Telomere length was measured in neutrophils and peripheral blood mononuclear cells (PBMC) at baseline and 8 weeks, with and without correction for cell counts. Main and interactive effects of n-3 fatty acids and CoQ on telomere length were assessed adjusting for baseline values. F2-isoprostanes were measured as markers of oxidative stress. There was no effect of n-3 fatty acids or CoQ on neutrophil or PBMC telomere length. However, telomere length corrected for neutrophil count was increased after n-3 fatty acids (p = 0.015). Post-intervention plasma F2-isoprostanes were negative predictors of post-intervention telomere length corrected for neutrophil count (p = 0.025).The effect of n-3 fatty acids to increased telomere length corrected for neutrophil count may relate to reduced oxidative stress and increased clearance of neutrophils with shorter telomeres from the circulation. This may be a novel mechanism of modifying CVD risk in CKD patients.

• Publisher: MDPI
• Subject: Adult / Aged / Antioxidants - adverse effects / Antioxidants - therapeutic use / Biomarkers - blood / blood plasma / cardiovascular diseases / coenzyme Q10 / Dietary Supplements - adverse effects / DNA / Docosahexaenoic Acids - adverse effects / Docosahexaenoic Acids - therapeutic use / Double-Blind Method / Drug Combinations / Eicosapentaenoic Acid - adverse effects / Eicosapentaenoic Acid - therapeutic use / F2-Isoprostanes - blood / Female / Humans / kidney diseases / Leukocytes, Mononuclear - drug effects / Leukocytes, Mononuclear - metabolism / Male / Middle Aged / mononuclear leukocytes / neutrophils / Neutrophils - drug effects / Neutrophils - metabolism / normal values / olive oil / omega-3 fatty acids / oxidative stress / Oxidative Stress - drug effects / patients / Renal Insufficiency, Chronic - diagnosis / Renal Insufficiency, Chronic - drug therapy / Renal Insufficiency, Chronic - genetics / Telomere - drug effects / Telomere - metabolism / Telomere Homeostasis - drug effects / telomeres / Time Factors / Treatment Outcome / Ubiquinone - adverse effects / Ubiquinone - analogs & derivatives / Ubiquinone - therapeutic use / Western Australia