Journal Article

Cytokine profiles in the aqueous humor following brolucizumab administration for exudative age-related macular degeneration

Okubo, Atsushi, Hashimoto, Yuto, Inoda, Satoru, Terao, Ryo, Aoki, Shuichiro, Takahashi, Hidenori, Misawa, Manami, Ahmed, Tazbir, Takahashi, Ryota, Azuma, Keiko, Obata, Ryo, Yoshida, Hana, Takahashi, Hironori

2023

Overview

PurposeTo identify the inflammatory cytokine profile in the aqueous humor (AH) of patients with intraocular inflammation (IOI) after intravitreal administration of brolucizumab (IVBr) for neovascular age-related macular degeneration.MethodsEight eyes from seven patients with IOI after initial IVBr (IVBrIOI +) were enrolled. Sixteen eyes from 16 patients without IOI after IVBr (IVBrIOI −) and aflibercept (IVA) were used as controls. AH samples were analyzed using a multiplex immunoassay.ResultsC–C motif chemokine ligand (CCL)2, C-X-C motif chemokine ligand (CXCL)1, CXCL10, CXCL13, interleukin (IL)-6, IL-8, IL-10, matrix metalloproteinase (MMP)-1, MMP-9, granulocyte colony-stimulating factor (G-CSF), granulocyte–macrophage colony-stimulating factor (GM-CSF), intercellular adhesion molecule (ICAM)-1, E-selectin, and P-selectin levels were significantly higher in IVBrIOI + than in IVBrIOI − and IVA. Vascular endothelial growth factor (VEGF) was significantly lower in IVBrIOI − compared to that in IVBrIOI + and IVA. In the IVBrIOI + group, there were significant correlations between CCL2, CXCL1, IL-6, IL-8, IL-10, G-CSF, GM-CSF, ICAM-1, and E-selectin, which also exhibited significant correlations in the IVBrIOI − group.ConclusionThe number of inflammatory cytokines increases during IOI, which is associated with type IV hypersensitivity and vascular inflammation. Some cytokines exhibit correlations even in non-inflamed eyes, indicating a subclinical response to IVBr.

• Publisher: Springer Nature B.V
• Subject: Age / Cell adhesion / Chemokines / Colonies / Colony-stimulating factor / CXCL10 protein / CXCL13 protein / Cytokines / E-selectin / Gelatinase B / Granulocyte colony-stimulating factor / Granulocyte-macrophage colony-stimulating factor / Granulocytes / Hypersensitivity / Inflammation / Intercellular adhesion molecule 1 / Interleukin 10 / Interleukin 6 / Interleukin 8 / Ligands / Macular degeneration / Matrix metalloproteinase / Metalloproteinase / Monoclonal antibodies / Monocyte chemoattractant protein 1 / Ophthalmology / P-selectin / Vascular endothelial growth factor