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Colorimetric determination of cysteine based on Au@Pt nanoparticles as oxidase mimetics with enhanced selectivity
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Colorimetric determination of cysteine based on Au@Pt nanoparticles as oxidase mimetics with enhanced selectivity
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Journal Article
Colorimetric determination of cysteine based on Au@Pt nanoparticles as oxidase mimetics with enhanced selectivity
2022
Overview
A H
2
O
2
-free colorimetric protocol based on urchin-like Au@Pt nanoparticles (Au@Pt NPs) has been developed for the sensitive and selective determination of cysteine (Cys). We verified the intrinsic oxidase-like activity of the Au@Pt NPs. They can act as artificial mimic oxidases to catalyse the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) with the assistance of dissolved oxygen, avoiding the use of H
2
O
2
in the colorimetric determination of Cys. In addition, the discrimination of Cys from the other two biothiol analogues, homocysteine and glutathione, can be easily realized through a simple ageing process. HNO
3
is added to this colorimetric system to terminate the reaction by oxidizing ox-TMB (oxidized form of TMB) to diphenoquinone (DPQ), thus generating a characteristic absorption peak of DPQ at 450 nm. By recording the absorbance at 450 nm, interference from the aggregated Au@Pt NPs (absorption peak at 670 nm) when 650 nm (the characteristic absorption peak of ox-TMB) is used as the absorption wavelength can be eliminated. We investigated this H
2
O
2
-free colorimetric protocol and obtained high sensitivity, with a detection limit of 1.5 nM and relatively high selectivity. The analytical performance for real samples was further explored. The Au@Pt NP–based H
2
O
2
-free colorimetric protocol is of great significance for the sensitive and selective determination of Cys in practical samples in different scenarios.
Graphical abstract
Publisher
Springer Vienna,Springer,Springer Nature B.V
Subject
