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An integrated magnetic separation enzyme-linked colorimetric sensing platform for field detection of Escherichia coli O157: H7 in food
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An integrated magnetic separation enzyme-linked colorimetric sensing platform for field detection of Escherichia coli O157: H7 in food
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Journal Article
An integrated magnetic separation enzyme-linked colorimetric sensing platform for field detection of Escherichia coli O157: H7 in food
2024
Overview
An intelligent colorimetric sensing platform integrated with in situ immunomagnetic separation function was developed for ultrasensitive detection of
Escherichia coli
O157: H7 (
E. coli
O157: H7) in food. Captured antibody modified magnetic nanoparticles (cMNPs) and detection antibody/horseradish peroxidase (HRP) co-functionalized AuNPs (dHAuNPs) were firstly synthesized for targeted enrichment and colorimetric assay of
E. coli
O157: H7, in which remarkable signal amplification was realized by loading large amounts of HRP on the surface of AuNPs. Coupling with the optical collimation attachments and embedded magnetic separation module, a highly integrated optical device was constructed, by which in situ magnetic separation and high-quality imaging of 96-well microplates containing
E. coli
O157: H7 was achieved with a smartphone. The concentration of
E. coli
O157: H7 could be achieved in one-step by performing digital image colorimetric analysis of the obtained image with a custom-designed app. This biosensor possesses high sensitivity (1.63 CFU/mL), short detecting time (3 h), and good anti-interference performance even in real-sample testing. Overall, the developed method is expected to be a novel field detection platform for foodborne pathogens in water and food as well as for the diagnosis of infections due to its portability, ease of operation, and high feasibility.
Graphical Abstract
Publisher
Springer Vienna,Springer,Springer Nature B.V
Subject
/ Antibodies, Immobilized - chemistry
/ Antibodies, Immobilized - immunology
/ Bacteria
/ Biosensing Techniques - methods
/ Characterization and Evaluation of Materials
/ Chemistry and Materials Science
/ E coli
/ Enzymes
/ Escherichia coli O157 - isolation & purification
/ Food
/ Food contamination & poisoning
/ Food Contamination - analysis
/ Gold
/ Horseradish Peroxidase - chemistry
/ Immunomagnetic Separation - methods
/ Magnetite Nanoparticles - chemistry
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