Journal Article

Comprehensive speed breeding: a high‐throughput and rapid generation system for long‐day crops

Yuan, Xin, Wang, Zhaoyang, Song, Yixian, Duan, Xingyu, Li, Xiang, Hong, Dengfeng, Yang, Guangsheng, Wang, Pengfei, Wan, Lili

2022

Overview

[...]the target plants are transplanted into pots and cultivated in a growth chamber, in which cold air is directed to circulate from the roof to the air flue and pass the plants, finally flowing back to the roof (Figure 1b). Both harvested seeds displayed normal germination rates ranging from 90% to 100%. Since the CSB procedure failed to induce flowering of typical winter type canola variety Darmor-bzh, we further improved the light regime by adding 500 μmol/m2/s far-red light (Figure 1j). RNA sequencing of the penultimate leaf in ZS11 showed that additional far-red light in CSB significantly increased the expression of multiple activators of flowering, including the B. napus orthologues of PHYTOCHROME A, CONSTANTS, and PHYTOCHROME-INTERACTING FACTOR 4 (Figure 1n). [...]extra far-red light can collectively enhance the transcriptional levels of FLOWERING LOCUS T (FT) homologues at both stages, leading to earlier flowering than that in solo CSB.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc
• Subject: Brief Communication / Brief Communications / Canola / comprehensive speed breeding / Conflicts of interest / Crops / Crops, Agricultural - genetics / Flowering / Flowers & plants / Gene expression / Gene sequencing / Genomes / Germination / Growth chambers / high‐throughput / Homology / Light emitting diodes / light regime / long‐day crop / molecular breeding / Phytochrome A / Plant Breeding / Roofs / Seeds / Transcription / Triticum / Wheat