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Marsdenia tenacissima extract prevents the malignant progression of glioma through upregulating lncRNA MEG3 and SFRP1‐dependent inhibition of Wnt/β‐catenin pathway
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Marsdenia tenacissima extract prevents the malignant progression of glioma through upregulating lncRNA MEG3 and SFRP1‐dependent inhibition of Wnt/β‐catenin pathway
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Journal Article
Marsdenia tenacissima extract prevents the malignant progression of glioma through upregulating lncRNA MEG3 and SFRP1‐dependent inhibition of Wnt/β‐catenin pathway
2023
Overview
Background/Aim Recent studies have highlighted the tumor‐suppressive effect of Marsdenia tenacissima extract (MTE) on human cancers. This research unveils the potential impact of MTE on glioma and ascertains the relevant molecular mechanisms. Methods Glioma cells were treated with MTE, with normal human astrocytes (NHAs) as controls. A battery of function experiments, including the CCK‐8 viability test, colony formation assay, scratch migration assay, and Transwell invasion assay, was executed to address the responses of glioma cells to MTE treatment and gain or loss of function of lncMEG3, miR‐542‐3p, and SFRP1. FISH, RIP, and dual‐luciferase reporter assays were adopted for assessing gene interactions. U251‐GFP‐Luc cells were delivered into nude mice through intracranial injection to develop an orthotopic glioma model for in vivo validation. Results 200 mg/mL MTE could suppress the proliferating, migrating, and invading properties of glioma cells but not affect those of NHAs. MTE treatment enhanced the expression of lncMEG3, which competes with SFRP1 for binding miR‐542‐3p. SFRP1 could inactivate the Wnt/β‐catenin pathway. Animal experimentation substantiated the antitumor activity and mechanism of MTE in nude mice. Conclusions MTE suppresses glioma via the lncMEG3/miR‐542‐3p/SFRP1/Wnt/β‐catenin axis. These findings contribute to a theoretical basis for the use of MTE for glioma patients. Marsdenia tenacissima extract increases lncMEG3 expression to regulate miR‐542‐3p/SFRP1 and inhibit Wnt/β‐catenin signaling pathway, thereby suppressing glioma cell proliferation, migration, and invasion.
Publisher
John Wiley & Sons, Inc,John Wiley and Sons Inc
Subject
/ Animals
/ Cells
/ Ethanol
/ Gene Expression Regulation, Neoplastic
/ Genes
/ Glioma
/ Humans
/ Intercellular Signaling Peptides and Proteins - genetics
/ Intercellular Signaling Peptides and Proteins - metabolism
/ Intercellular Signaling Peptides and Proteins - pharmacology
/ Marsdenia tenacissima extract
/ Membrane Proteins - genetics
/ Membrane Proteins - metabolism
/ Mice
/ Original
/ Proteins
/ Reagents
/ RNA, Long Noncoding - genetics
/ SFRP1
/ Traditional Chinese medicine
/ Tumors
