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Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture
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Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture
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Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture
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Journal Article
Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture
2016
Overview
A method for rapid cloning of plant disease-resistance genes could provide sustainable, genetic solutions to crop pests and pathogens in place of agrichemicals.
Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5–15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution
1
. If several cloned R genes were available, it would be possible to pyramid R genes
2
in a crop, which might provide more durable resistance
1
. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes
Sr22
and
Sr45
from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 45/70
/ Barley
/ Biomedical Engineering/Biotechnology
/ Cloning
/ Cloning, Molecular - methods
/ Corn
/ Disease Resistance - genetics
/ Genes
/ Genetic Enhancement - methods
/ letter
/ Mutagenesis, Site-Directed - methods
/ Plant Diseases - prevention & control
/ Plants, Genetically Modified - genetics
/ Rice
/ Sequence Analysis, DNA - methods
/ Wheat
/ Zea mays
