Journal Article

Circulating microRNA profiles based on direct S‐Poly(T)Plus assay for detection of coronary heart disease

Su, Mingyang, Qu, Junle, Gou, Deming, Dang, Quanjin, Niu, Yanqin, Tang, Zhongren, Zhu, Daling

2020

Overview

Coronary heart disease (CHD) is one of the leading causes of heart‐associated deaths worldwide. Conventional diagnostic techniques are ineffective and insufficient to diagnose CHD with higher accuracy. To use the circulating microRNAs (miRNAs) as non‐invasive, specific and sensitive biomarkers for diagnosing of CHD, 203 patients with CHD and 144 age‐matched controls (126 high‐risk controls and 18 healthy volunteers) were enrolled in this study. The direct S‐Poly(T)Plus method was used to identify novel miRNAs expression profile of CHD patients and to evaluate their clinical diagnostic value. This method is an RNA extraction‐free and robust quantification method, which simplifies procedures, reduces variations, in particular increases the accuracy. Twelve differentially expressed miRNAs between CHD patients and high‐risk controls were selected, and their performances were evaluated in validation set‐1 with 96 plasma samples. Finally, six (miR‐15b‐5p, miR‐29c‐3p, miR‐199a‐3p, miR‐320e, miR‐361‐5p and miR‐378b) of these 12 miRNAs were verified in validation set‐2 with a sensitivity of 92.8% and a specificity of 89.5%, and the AUC was 0.971 (95% confidence interval, 0.948‐0.993, P < .001) in a large cohort for CHD patients diagnosis. Plasma fractionation indicated that only a small amount of miRNAs were assembled into EVs. Direct S‐Poly(T)Plus method could be used for disease diagnosis and 12 unique miRNAs could be used for diagnosis of CHD.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc
• Subject: Apolipoproteins / Biological Assay / biomarker / Biomarkers / Blood / Body mass index / Cardiovascular disease / Case-Control Studies / Circulating MicroRNA - blood / circulating miRNA / Cluster Analysis / Cohort Studies / Coronary artery disease / Coronary Disease - blood / Coronary Disease - diagnosis / Coronary Disease - genetics / coronary heart disease (CHD) / Coronary vessels / Diagnosis / direct S‐Poly(T)Plus / Female / Gene expression / Gene Expression Profiling / Gene Expression Regulation / Heart / Heart diseases / Hospitals / Humans / Lipoproteins / Male / Medical imaging / MicroRNAs / Middle Aged / miRNA / Original / Poly T - metabolism / Reproducibility of Results / reverse transcription quantitative polymerase chain reaction (RT‐qPCR) / Risk Factors / Veins & arteries