Asset Details
Do you wish to reserve the book?
Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol
Do you wish to request the book?
Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol
2006
Overview
Abstract
In this study, the M13 primer was used to distinguish Geotrichum candidum from the anamorphic and teleomorphic forms of other arthrospore-forming species (discriminatory power = 0.99). For intraspecific characterization, the GATA4 primer showed the highest level of discrimination for G. candidum among the 20 microsatellite primers tested. A molecular typing protocol (DNA concentration, hybridization temperature and type of PCR machine) was optimized through a series of intra- and interlaboratory trials. This protocol was validated using 75 strains of G. candidum, one strain of G. capitatum and one strain of G. fragrans, and exhibited a discrimination score of 0.87. This method could therefore be used in the agro-food industries to identify and to evaluate biodiversity and trace strains of G. candidum. The results show that the GATA4 primer might be used to differentiate strains according to their ecological niche.
Publisher
Oxford University Press,Springer
Subject
/ Bacteriophage M13 - genetics
/ Biological and medical sciences
/ Fundamental and applied biological sciences. Psychology
/ genetics
/ Geotrichum - isolation & purification
/ methods
/ Mycological Typing Techniques
/ Mycological Typing Techniques - methods
/ Mycological Typing Techniques - standards
/ Studies
/ yeasts
