Journal Article

How Do Different Dietary Carbohydrate/Lipid Ratios Influence Intestinal Morphology and Glycolipid Metabolism Capacity in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)

Zhang, Shuang, Yang, Qihui, Guo, Xinwei, Tan, Beiping, Yang, Xuanyi, Liu, Hongyu, Chi, Shuyan, Dong, Xiaohui

2023

Overview

Levels of dietary carbohydrates and lipids have important effects on the growth, health, and glycolipid metabolizing capacity of the hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂). This experiment evaluated the effect of carbohydrate to lipid ratios (CHO:L ratio) on growth, serum biochemical indices, intestinal morphology and activity of digestive enzymes, and the ability to metabolize carbohydrates and lipids in the hybrid grouper. Six iso-nitrogenous (500 g/kg) and iso-energetic (190 MJ/kg) feeds with CHO:L ratios of 0.82, 1.03, 1.28, 1.58, 1.94, and 2.27 were formulated. The 720 groupers with an initial body weight of 7.76 ± 0.08 g were sorted into 24 fiberglass buckets of 30 fish each. Feed was delivered twice daily at 8:00 and 16:00 to apparent satiety for 56 days. The results showed that the growth performance of the groupers was not significantly affected by the diet. Based on the specific growth rate (SGR), the optimal CHO:L ratio was 1.72 for the groupers by the second-order polynomial regression model. The intestinal trypsin and lipase, and the hepatic trypsin activities of the groupers showed a maximum in the 1.58 group. The intestinal muscle thickness in the 1.94 group and the villus length in the 1.58 group were significantly greater than in the 0.82, 1.03, 1.28, and 2.27 groups. The glycogen synthase, pyruvate kinase, and hexokinase activities of the liver were significantly higher in the 1.58 group than in the 0.82 and 2.27 groups. The hormone-sensitive triglyceride lipase, adipose triglyceride lipase, acetyl-CoA carboxylase, and carnitine palmitoyl transferase of the liver showed significantly higher activities in the 1.58 group than those in the 0.82 and 1.03 groups. These results showed that based on the SGR of the groupers, the best CHO:L ratio was 1.72. An appropriate dietary CHO:L ratio can reduce the lipid deposition of the fish body, liver, and muscle, as well as improve the intestinal morphology and activities of liver carbohydrate and lipid metabolism enzymes.

• Publisher: MDPI AG
• Subject: Acetyl-CoA carboxylase / Aquaculture / Bioengineering / blood serum / Body weight / carbohydrate and lipid metabolism / Carbohydrate metabolism / carbohydrate to lipid ratios / Carbohydrates / Carnitine / carnitine palmitoyltransferase / dietary carbohydrate / Digestive enzymes / Energy / Enzymes / Epinephelus / Epinephelus fuscoguttatus / Fiberglass / Fish / Glucose / Glycogen / glycogen (starch) synthase / Glycogen synthase kinase 3 / Glycolipids / grouper / growth performance / Growth rate / Hexokinase / Hormones / hybrids / intestinal morphology / Intestine / intestines / Kinases / Lipase / Lipid metabolism / Lipids / Liver / Marine fishes / Metabolism / Morphology / muscles / Nitrogen / Nutrition / Proteins / Pyruvate kinase / Pyruvic acid / Ratios / regression analysis / Satiety / Serum / Soybeans / specific growth rate / Synthesis / triacylglycerol lipase / Triglycerides / Trypsin / villi / Villus / Whitefish