Journal Article

The Plant Proteinase Inhibitor CrataBL Plays a Role in Controlling Asthma Response in Mice

Righetti, Renato F., Ferreira, Rodrigo da Silva, Oliva, Maria Luiza Vilela, Martins, Milton Arruda, Ruiz-Schutz, Viviane Christina, Brito, Marlon Vilela de, Brüggemann, Thayse Regina, Paiva, Patrícia Maria Guedes, Souza, Flávia Castro Ribas de, Correia, Maria Tereza dos Santos, Leick, Edna Aparecida, Rodrigues, Adriana Palmeira Dias, Arantes-Costa, Fernanda Magalhães, Bortolozzo, Anelize Sartori Santos, Tibério, Iolanda F. L. C., Prado, Carla M., Saraiva-Romanholo, Beatriz Mangueira

2018

Overview

Background. CrataBL is a protein isolated from Crataeva tapia bark. It has been shown to exhibit several biological properties, including anti-inflammatory, analgesic, antitumor, and insecticidal activities. There are no studies evaluating the role of CrataBL in experimental asthma models. Aim. To evaluate the effects of CrataBL on lung mechanics, inflammation, remodeling, and oxidative stress activation of mice with allergic pulmonary inflammation. Materials and Methods. BALB/c mice (6-7 weeks old, 25-30g) were divided into four groups: nonsensitized and nontreated mice (C group, n=8); ovalbumin- (OVA-) sensitized and nontreated mice (OVA group, n=8); nonsensitized and CrataBL-treated mice (C+CR group, n=8); OVA-sensitized and CrataBL-treated mice (OVA+CR group, n=8). We evaluated hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), pulmonary inflammation, extracellular matrix remodeling, and oxidative stress markers. Results. CrataBL treatment in OVA-sensitized mice (OVA+CR group) attenuated the following variables compared to OVA-sensitized mice without treatment (OVA group) (all p<0.05): (1) respiratory system resistance (Rrs) and elastance (Ers) after methacholine challenge; (2) total cells, macrophages, polymorphonuclear cells, and lymphocytes in BALF; (3) eosinophils and volume fraction of collagen and elastic fibers in the airway and alveolar wall according to histopathological and morphometry analysis; (4) IL-4-, IL-5-, IL-13-, IL-17-, IFN-γ-, MMP-9-, TIMP-1-, TGF-β-, iNOS-, and NF-kB-positive cells and volume of 8-iso-PGF2α in airway and alveolar septa according to immunohistochemistry; and (5) IL-4, IL-5, and IFN-γ according to an ELISA. Conclusion. CrataBL contributes to the control of hyperresponsiveness, pulmonary inflammation, extracellular matrix remodeling, and oxidative stress responses in an animal model of chronic allergic pulmonary inflammation.

• Publisher: Hindawi Publishing Corporation,Hindawi,John Wiley & Sons, Inc
• Subject: Albumin / Allergies / Alveoli / Analgesics / Analysis / Animal models / Animals / Anticancer properties / Asthma / Asthma - drug therapy / Asthma - metabolism / Bark / Biological products industry / Biological properties / Bronchial Provocation Tests - methods / Bronchoalveolar Lavage Fluid - chemistry / Bronchus / Capparaceae - chemistry / Collagen / Crataeva tapia / Disease Models, Animal / Enzyme Inhibitors - pharmacology / Enzyme-linked immunosorbent assay / Eosinophils / Eosinophils - drug effects / Eosinophils - metabolism / Extracellular matrix / Fibers / Gelatinase B / Hypersensitivity - drug therapy / Hypersensitivity - metabolism / Immunohistochemistry / Inflammation / Insecticide resistance / Interleukin 13 / Interleukin 17 / Interleukin 4 / Interleukin 5 / Interleukin-13 - metabolism / Interleukin-4 - metabolism / Laboratory animals / Leukocytes (eosinophilic) / Leukocytes (polymorphonuclear) / Lung - drug effects / Lung - metabolism / Lungs / Lymphocytes / Macrophages / Male / Mass spectrometry / Methacholine / Mice / Mice, Inbred BALB C / Nitric oxide / Ovalbumin / Ovalbumin - pharmacology / Oxidative stress / Plant Lectins - pharmacology / Plant Proteins - pharmacology / Pneumonia - drug therapy / Pneumonia - metabolism / Proteinase / Proteinase inhibitors / Proteins / Respiratory system / Respiratory tract / Scientific imaging / Septum / Type 2 diabetes / γ-Interferon