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Mutation of the Plastidial α-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm
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Mutation of the Plastidial α-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm
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Mutation of the Plastidial α-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm
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Journal Article
Mutation of the Plastidial α-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm
2008
Overview
Plastidial phosphorylase (Pho1) accounts for ~96% of the total phosphorylase activity in developing rice (Oryza sativa) seeds. From mutant stocks induced by N-methyl-N-nitrosourea treatment, we identified plants with mutations in the Pho1 gene that are deficient in Pho1. Strikingly, the size of mature seeds and the starch content in these mutants showed considerable variation, ranging from shrunken to pseudonormal. The loss of Pho1 caused smaller starch granules to accumulate and modified the amylopectin structure. Variation in the morphological and biochemical phenotype of individual seeds was common to all 15 pho1-independent homozygous mutant lines studied, indicating that this phenotype was caused solely by the genetic defect. The phenotype of the pho1 mutation was temperature dependent. While the mutant plants grown at 30°C produced mainly plump seeds at maturity, most of the seeds from plants grown at 20°C were shrunken, with a significant proportion showing severe reduction in starch accumulation. These results strongly suggest that Pho1 plays a crucial role in starch biosynthesis in rice endosperm at low temperatures and that one or more other factors can complement the function of Pho1 at high temperatures.
Publisher
American Society of Plant Biologists
Subject
/ Enzymes
/ Gels
/ Gene Expression Regulation, Enzymologic
/ Gene Expression Regulation, Plant
/ genes
/ Grains
/ Microscopy, Electron, Scanning
/ mutants
/ Mutation
/ Plants, Genetically Modified - enzymology
/ Plants, Genetically Modified - genetics
/ Plants, Genetically Modified - metabolism
/ Plants, Genetically Modified - ultrastructure
/ Reverse Transcriptase Polymerase Chain Reaction
/ Rice
/ Seeds
/ Starches
