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Broad-spectrum antimicrobial activity and high stability of Trichokonins from Trichoderma koningii SMF2 against plant pathogens
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Broad-spectrum antimicrobial activity and high stability of Trichokonins from Trichoderma koningii SMF2 against plant pathogens
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Journal Article
Broad-spectrum antimicrobial activity and high stability of Trichokonins from Trichoderma koningii SMF2 against plant pathogens
2006
Overview
Abstract
Antimicrobial metabolites produced by Trichoderma koningii SMF2 exhibited antimicrobial activity against a range of Gram-positive bacterial and fungal phytopathogens. Purification of these metabolites was achieved using combinations of gel filtration and high-performance liquid chromatography. Identified by liquid chromatography electrospray ionization tandem mass spectrometry, the active metabolites proved to be three known peptaibols: Trichokonin VI, VII and VIII. The Trichokonins were stable and remained biological active over a wide pH range and at every temperature tested, showing no loss of activity even after autoclaving. Trichokonins were insensitive to proteolytic enzymes. Trichokonin VI takes on typical helical structure and the structure changes only slightly at different temperatures and pH values. The present study presented the potential of Trichokonins to be used as biological control agents.
Publisher
Blackwell Publishing Ltd,Blackwell,Oxford University Press
Subject
Action of physical and chemical agents
/ Action of physical and chemical agents on bacteria
/ Alamethicin - analogs & derivatives
/ Alamethicin - isolation & purification
/ Anti-Infective Agents - pharmacology
/ Antiinfectives and antibacterials
/ Biological and medical sciences
/ Chromatography, High Pressure Liquid
/ Fundamental and applied biological sciences. Psychology
/ High-performance liquid chromatography
/ Ions
/ Mycology
/ Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
