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Characterization of alphabaculovirus: HearNPV-IIPR05 isolate infecting Helicoverpa armigera (Hubner) larvae
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Characterization of alphabaculovirus: HearNPV-IIPR05 isolate infecting Helicoverpa armigera (Hubner) larvae
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Characterization of alphabaculovirus: HearNPV-IIPR05 isolate infecting Helicoverpa armigera (Hubner) larvae
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Journal Article
Characterization of alphabaculovirus: HearNPV-IIPR05 isolate infecting Helicoverpa armigera (Hubner) larvae
2021
Overview
Background
The alphabaculoviruses are lethal pathogens of lepidopteran caterpillars including a polyphagous and globally recognized pest,
Helicoverpa armigera
(Hubner) infesting economically important agriculture crops worldwide. The biological and molecular characterizations of indigenous
nucleopolyhedrovirus
of the genus
Alphabaculovirus
isolated from
H. armigera
in chickpea fields are described.
Results
The virulence of virus isolate was tested in 3rd instar
H. armigera
larvae, and LC
50
(median lethal concentration) was estimated to be 2.69 × 10
4
OBs ml
−1
. The ST
50
(median survival time) was 4 days post-inoculation, when the 3rd instar
H. armigera
larvae were inoculated by OB (occlusion body) concentration equivalent to LC
90
. An average incubation period of the virus isolate in 3rd instar ranged between 4 and 6 days post-inoculation. The OBs of a virus isolate appeared irregular in shape and variable in size with diameter ranging from 0.57 to 1.46 μm on the longest edge and average of 1.071 ± 0.068 μm (mean ± SE). On the basis of phylogenetic analysis of
polh
,
pif-1
, and
lef-8
genes, the isolate was found to be a member of the genus
Alphabaculovirus.
The isolate showed a genetic affinity with species of group II
Alphabaculoviruses
and appeared to be a group II NPV.
Conclusions
On the basis of molecular phylogeny and associated host insect, this indigenous isolate was designated as HearNPV-IIPR05 isolate, which could be a potential candidate for the biological control of
H. armigera
infesting legumes and other commercial crops.
