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Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry
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Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry
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Journal Article
Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry
2020
Overview
We demonstrate single-particle charge detection mass spectrometry on an Orbitrap for the analysis of megadalton biomolecular assemblies. We establish that the signal amplitudes of individual ions scale linearly with their charge, which can be used to resolve mixed ion populations, determine charge states and thus also determine the masses of individual ions. This enables the ultrasensitive analysis of heterogeneous protein assemblies including immunoglobulin oligomers, ribosomes, proteinaceous nanocontainers and genome-packed adeno-associated viruses.
An Orbitrap-based method for single-particle charge detection mass spectrometry enables facile, ultrasensitive analysis of protein assemblies.
Publisher
Nature Publishing Group US,Nature Publishing Group
