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Generation of novel polyclonal antibodies against Mycobacterium tuberculosis lipoarabinomannan, EspB, and Mtb8
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Generation of novel polyclonal antibodies against Mycobacterium tuberculosis lipoarabinomannan, EspB, and Mtb8
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Generation of novel polyclonal antibodies against Mycobacterium tuberculosis lipoarabinomannan, EspB, and Mtb8
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Journal Article
Generation of novel polyclonal antibodies against Mycobacterium tuberculosis lipoarabinomannan, EspB, and Mtb8
2025
Overview
The unique cell wall of
Mycobacterium tuberculosis
(Mtb) creates a barrier to hydrophilic drugs, which is crucial for its survival and pathogenicity. However, the immune reactivity elicited by its components remains incompletely understood. We aimed to assess the antibody responses induced by
Mtb H37Rv
cell wall components and to develop and characterize antigen-specific polyclonal antibodies (pAbs). Rabbits were immunized with these components. Immune serum reactivity was tested against various Mtb antigens. Specific polyclonal antibodies (pAbs) were purified by affinity chromatography. The results showed that immune serum reacted with lipoarabinomannan (LAM), ESAT-6 secretion system-1 (Esx-1) secreted protein B (EspB), and Mtb8, but showed no reactivity with other tested Mtb antigens. LAM-, EspB-, or Mtb8-specific pAbs were subsequently affinity-purified. The affinity-purified LAM pAb, EspB pAb, and Mtb8 pAb each demonstrated high specificity and sensitivity, showing no cross-reactivity with non-target antigens. They recognized antigens in culture supernatants and cells from diverse mycobacterial strains, including both slow-growing mycobacteria (SGM) and rapid-growing mycobacteria (RGM). In a sandwich ELISA using LAM pAb as the capture antibody and biotinylated LAM-specific monoclonal Abs (BJRbL01-Bio, BJRbL03-Bio, BJRbL20-Bio, BJRbL52-Bio, or BJRbL76-Bio) as detection antibodies, the assay detected SGM but did not react with RGM species. EspB pAb recognized EspB in both cell lysate and culture supernatant fractions, where full-length and mature EspB are predominantly found, respectively. Mtb8 pAb reacted with monomeric and polymeric forms of Mtb8. In conclusion, we successfully generated novel pAbs against LAM, EspB, and Mtb8, providing promising research tools for investigating these critical molecules.
Key points
Rabbit antibodies against Mtb H37Rv cell wall components target LAM, EspB, and Mtb8
Novel LAM-, EspB-, and Mtb8-specific pAbs were generated and characterized
Broad mycobacterial reactivity and specific target detection confirm pAb utility
Publisher
Springer Berlin Heidelberg,Springer Nature B.V,Springer
Subject
/ Animals
/ Antibodies, Bacterial - immunology
/ Antibodies, Bacterial - isolation & purification
/ Antigens
/ Antigens, Bacterial - immunology
/ Antisera
/ Bacterial Proteins - immunology
/ Biomedical and Life Sciences
/ Enzyme-Linked Immunosorbent Assay
/ EspB
/ LAM
/ Lipopolysaccharides - immunology
/ Microbial Genetics and Genomics
/ Mtb8
/ Mycobacterium tuberculosis - immunology
/ polymers
/ Proteins
/ Rabbits
/ species
/ Vaccines
