Journal Article

Plasma Peptide Concentrations and Peptide-Reactive Immunoglobulins in Patients with Eating Disorders at Inclusion in the French EDILS Cohort (Eating Disorders Inventory and Longitudinal Survey)

Le Solliec, Marie-Anne, Rimbert, Agnès, Lucas, Nicolas, Saillard, Tiphaine, Petit, André, Déchelotte, Pierre, Grigioni, Sébastien, Folope, Vanessa, Galmiche, Marie, Azhar, Saida, Achamrah, Najate, Gillibert, André, Rondeaux, Julie, Tavolacci, Marie-Pierre, Lamarre, Adèle, Lambert, Grégory, Quillard, Muriel, Belmonte, Liliana, Coeffier, Moise, Legrand, Romain, Deroissart, Camille, Colange, Guillaume, Delay, Julie

2020

Overview

Eating disorders (EDs) are increasingly frequent. Their pathophysiology involves disturbance of peptide signaling and the microbiota–gut–brain axis. This study analyzed peptides and corresponding immunoglobulin (Ig) concentrations in groups of ED. In 120 patients with restrictive (R), bulimic (B), and compulsive (C) ED, the plasma concentrations of leptin, glucagon-like peptide-1 (GLP-1), peptide YY (PYY), and insulin were analyzed by Milliplex and those of acyl ghrelin (AG), des-acyl ghrelin (DAG), and α-melanocyte-stimulating hormone (α-MSH) by ELISA kits. Immunoglobulin G (in response to an antigen) concentrations were analyzed by ELISA, and their affinity for the respective peptide was measured by surface plasmon resonance. The concentrations of leptin, insulin, GLP-1, and PYY were higher in C patients than in R patients. On the contrary, α-MSH, DAG, and AG concentrations were higher in R than in C patients. After adjustment for body mass index (BMI), differences among peptide concentrations were no longer different. No difference in the concentrations of the IgG was found, but the IgG concentrations were correlated with each other. Although differences of peptide concentrations exist among ED subtypes, they may be due to differences in BMI. Changes in the concentration and/or affinity of several anti-peptide IgG may contribute to the physiopathology of ED or may be related to fat mass.

• Publisher: MDPI AG,MDPI
• Subject: antigens / Body Mass Index / Classification / Cohort Studies / Eating behavior / Eating disorders / Enzyme-Linked Immunosorbent Assay / Enzymes / Feeding and Eating Disorders - immunology / Female / Food / Food and Nutrition / France / ghrelin / glucagon-like peptide 1 / Glucagon-Like Peptide 1 - blood / Gut-brain axis / Humans / Immune system / immunoglobulin G / Immunoglobulin G - blood / Insulin / Insulin - blood / inventories / leptin / Leptin - blood / Life Sciences / Longitudinal Studies / Male / mass / Microbiota / nutrients / pathophysiology / patients / peptide YY / Peptide YY - blood / Peptides / Peptides - blood / Peptides - immunology / Plasma / Questionnaires / surface plasmon resonance