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High‐Throughput Immunoassays for Cavin‐4 IgG: A Diagnostic Tool for Immune‐Mediated Rippling Muscle Disease
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High‐Throughput Immunoassays for Cavin‐4 IgG: A Diagnostic Tool for Immune‐Mediated Rippling Muscle Disease
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Journal Article
High‐Throughput Immunoassays for Cavin‐4 IgG: A Diagnostic Tool for Immune‐Mediated Rippling Muscle Disease
2025
Overview
ABSTRACT
Cavin‐4 was identified as a potential autoantigen for immune‐mediated rippling muscle disease (iRMD). To validate this, we developed and tested various immunoassays, including a cell‐based assay (CBA), cavin‐4 recombinant protein ELISA, and multi‐peptide ELISA. Among 19 iRMD patients, all exhibited muscle rippling, and 13 had percussion‐induced mounding. All immunoassays demonstrated clinical and analytical specificities greater than 95%. The protein ELISA had the highest sensitivity (94.7%) and specificity (99.9%), outperforming CBA (sensitivity 89.5%, specificity 99.6%) and the multi‐peptide ELISA (sensitivity 79.0%, specificity 97.2%). Our results suggest that the cavin‐4 protein ELISA is a promising tool for high‐throughput clinical testing in iRMD.
Publisher
John Wiley & Sons, Inc,John Wiley and Sons Inc,Wiley
Subject
/ Adult
/ Antigens
/ caveolae‐associated protein 4 (cavin‐4)
/ Child
/ Enzyme-Linked Immunosorbent Assay - methods
/ Enzyme-Linked Immunosorbent Assay - standards
/ enzyme‐linked immunosorbent assay (ELISA)
/ Female
/ High-Throughput Screening Assays - methods
/ Humans
/ immune‐mediated rippling muscle disease (iRMD)
/ Kinases
/ Male
/ Muscular Diseases - diagnosis
/ Muscular Diseases - immunology
/ Patients
/ Peptides
/ phage immunoprecipitation sequencing (PhIP‐Seq)
/ Proteins
