Journal Article

A Single Species of Clostridium Subcluster XIVa Decreased in Ulcerative Colitis Patients

Sato, Tadashi, Mikami, Yohei, Naganuma, Makoto, Matsuoka, Katsuyoshi, Takada, Toshihiko, Nomoto, Koji, Kanai, Takanori, Takeshita, Kozue, Saigusa, Keiichiro, Kushiro, Akira, Mizuno, Shinta, Sujino, Tomohisa, Tsuji, Hirokazu

2016

Overview

Imbalance of the intestinal microbiota is associated with gastrointestinal disease and autoimmune disease and metabolic syndrome. Analysis of the intestinal microbiota has recently progressed, and the association with inflammatory bowel disease has been reported at the species level. Such findings suggest that the recovery of homeostasis in the intestinal microbiota could cure inflammatory bowel disease. We aimed to search new probiotic candidates for inflammatory bowel disease through translational research by analysis of ulcerative colitis (UC) patients' intestinal microbiota and clarify the effects of them on inflammation. Here, we focused on Fusicatenibacter saccharivorans, which belongs to Clostridium subcluster XIVa and was successfully isolated and cultured in 2013. We analyzed the association of F. saccharivorans to UC patients' activity and inflammation for the first time.MethodsFeces from UC patients and healthy controls were analyzed by 16S ribosomal RNA gene sequences. F. saccharivorans was administered to murine colitis model. Colitic lamina propria mononuclear cells from UC patients and mice were stimulated with F. saccharivorans.ResultsThe whole fecal bacteria in active UC patients were less than that in quiescent UC patients. Furthermore, F. saccharivorans was decreased in active UC patients and increased in quiescent. The administration of F. saccharivorans improved murine colitis. F. saccharivorans induced interleukin 10 production by lamina propria mononuclear cells from not only colitis model mice but also UC patients.Conclusions F. saccharivorans decreased in correlation to UC activity and suppresses intestinal inflammation. These results suggest that F. saccharivorans could lead to a novel UC treatment.

• Publisher: Oxford University Press
• Subject: Adult / Aged / Animals / Case-Control Studies / Clostridium / Clostridium - genetics / Clostridium - physiology / Colitis, Ulcerative - microbiology / Disease Progression / Feces - microbiology / Female / Gastrointestinal Microbiome - physiology / Humans / Inflammation / Inflammation - microbiology / Inflammatory bowel disease / Intestinal Mucosa - microbiology / Male / Mice / Mice, Inbred C57BL / Microbiota / Middle Aged / Mucous Membrane - microbiology / RNA, Ribosomal, 16S - analysis / Species Specificity / Young Adult