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Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted
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Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted
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Journal Article
Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted
2017
Overview
Diacetyl causes an unwanted buttery off-flavor in lager beer. The production of diacetyl is reduced by modifying the metabolic pathway of yeast in the beer fermentation process. In this study,
BDH2
and
ILV5
genes, coding diacetyl reductase and acetohydroxy acid reductoisomerase, respectively, were expressed using a
PGK
1 promoter in
Saccharomyces cerevisiae
, which deleted one
ILV2
allelic gene. Diacetyl contents and fermentation performances were examined and compared. Results showed that the diacetyl content in beer was remarkably reduced by 16.52% in QI2-KP (one
ILV2
allelic gene deleted), 55.65% in QI2-B2Y (overexpressed
BDH2
gene and one
ILV2
allelic gene deleted), and 69.13% in QI2-I5Y (overexpressed
ILV5
gene and one
ILV2
allelic gene deleted) compared with the host strain S2. The fermentation ability of mutant strains was similar to that of S2. Results of the present study can lead to further advances in this technology and its broad application in scientific investigations and industrial beer production.
Publisher
Springer Berlin Heidelberg,Oxford University Press
Subject
Alcohol Oxidoreductases - genetics
/ Alcohol Oxidoreductases - metabolism
/ Alleles
/ Beer
/ beers
/ Biomedical and Life Sciences
/ Cell Culture and Bioengineering - Original Paper
/ Cloning
/ diacetyl
/ E coli
/ Genes
/ Kinases
/ Mitochondrial Proteins - genetics
/ Mitochondrial Proteins - metabolism
/ mutants
/ Plasmids
/ Saccharomyces cerevisiae - genetics
/ Saccharomyces cerevisiae - metabolism
/ Saccharomyces cerevisiae Proteins - genetics
/ Saccharomyces cerevisiae Proteins - metabolism
/ Studies
/ Yeast
/ yeasts
