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Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems
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Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems
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Journal Article
Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems
2024
Overview
Multicellular systems grow over the course of weeks from single cells to tissues or even full organisms, making live imaging challenging. To bridge spatiotemporal scales, we present an open-top dual-view and dual-illumination light-sheet microscope dedicated to live imaging of large specimens at single-cell resolution. The configuration of objectives together with a customizable multiwell mounting system combines dual view with high-throughput multiposition imaging. We use this microscope to image a wide variety of samples and highlight its capabilities to gain quantitative single-cell information in large specimens such as mature intestinal organoids and gastruloids.
This work presents a highly versatile open-top, dual-view and dual-illumination light-sheet microscope for live imaging of large specimens.
Publisher
Nature Publishing Group US,Nature Publishing Group
