Journal Article

Anti-wrinkle and anti-whitening effects of jucá (Libidibia ferrea Mart.) extracts

Smith, Marilia de Arruda Cardoso, Silva, Felipe Moura Araújo da, Nogueira, Jéssica Rodrigues, Fruet, Andréa Costa, Maria-Engler, Silvya Stuchi, de Souza, Tatiane Pereira, Calcagno, Danielle Queiroz, Pedrosa, Tatiana do Nascimento, Rodrigues, Isis Costa, Koolen, Hector Henrique Ferreira, Barros, Aline Oliveira, Lima, Emerson Silva, de Vasconcellos, Marne Carvalho, Barros, Silvia Berlanga de Moraes

2016

Overview

Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea , popularly known as jucá , is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of jucá trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC–MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC 50  = 8.5 ± 0.8 µg/mL) and LFP (IC 50  = 16 ± 0.5 µg/mL), was stronger than standard rutin (IC 50  = 27.6 ± 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.

• Publisher: Springer Berlin Heidelberg,Springer Nature B.V
• Subject: Aging / Animals / Antioxidants / Antioxidants - pharmacology / Caesalpinia - chemistry / Cell Line, Tumor / Cell Survival / Cell viability / Collagenase / Colorimetry / Cosmetics - pharmacology / Dermatology / Elastase / Enzymatic activity / Enzyme Precursors - metabolism / Enzymes / Fibroblasts / Flavonoids / Flavonoids - pharmacology / Gelatinase A / Gelatinase B / Gelatinases - metabolism / Humans / Hyaluronic acid / Libidibia ferrea / Liquid chromatography / Mass spectroscopy / Matrix metalloproteinase / Matrix Metalloproteinase 2 - metabolism / Matrix Metalloproteinase 9 - metabolism / Medicine / Medicine & Public Health / Melanin / Melanins - metabolism / Metalloproteinase / Mice / Monophenol Monooxygenase - metabolism / Original Paper / Phenolic acids / Phenols - pharmacology / Phytochemicals / Plant Bark / Plant Extracts - pharmacology / Primary Cell Culture / Rutin / Skin / Skin Aging - drug effects / Solvents / Tandem Mass Spectrometry