Journal Article

A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate

Tateno, Mana, Iwasaki, Yu, Shigemura, Yasutaka, Suzuki, Asahi, Sato, Kenji, Kurokawa, Mihoko, Sato, Yoshio

2018

Overview

Levels of short linear hydroxyproline (Hyp)-containing peptides, such as prolyl-hydroxyproline (Pro-Hyp), increase in human blood after the ingestion of collagen hydrolysate, which has been associated with beneficial effects for human skin and joints. The present study demonstrates the presence of a novel food-derived collagen peptide, cyclic Pro-Hyp, in human blood after the ingestion of collagen hydrolysate. The cyclic Pro-Hyp levels in plasma samples were estimated by liquid chromatography mass spectrometry (LC-MS). Cyclic Pro-Hyp levels significantly increased in the plasma after ingestion of collagen hydrolysate, reaching a maximum level after 2 h and then decreasing. The maximum level of cyclic Pro-Hyp in plasma ranged from 0.1413 to 0.3443 nmol/mL, representing approximately 5% of linear Pro-Hyp in plasma after ingestion of collagen hydrolysate. Addition of cyclic Pro-Hyp in medium at 7 nmol/mL significantly enhanced the growth rate of mouse skin fibroblasts on collagen gel more extensively compared to linear Pro-Hyp.

• Publisher: MDPI
• Subject: Adult / Animals / blood / collagen / Collagen - administration & dosage / Collagen - blood / Collagen - pharmacology / Dipeptides - blood / Eating / Female / fibroblasts / Fibroblasts - drug effects / Gelatin / gels / Humans / hydrolysates / hydroxyproline / Hydroxyproline - blood / liquid chromatography / mass spectrometry / Mice / Middle Aged / peptides / Peptides - administration & dosage / Peptides - blood / Peptides - pharmacology / Pilot Projects / Protein Hydrolysates - administration & dosage / Protein Hydrolysates - blood / Protein Hydrolysates - pharmacology / protein intake / Skin / skin (animal) / Sus scrofa / Tandem Mass Spectrometry