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The bacterial replication origin BUS promotes nucleobase capture
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Journal Article
The bacterial replication origin BUS promotes nucleobase capture
2023
Overview
Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at the chromosome origin (
oriC
) that engages both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to promote DNA duplex opening. However, the mechanism of DnaA specifically opening a replication origin was unknown. Here we show that
Bacillus subtilis
DnaA
ATP
assembles into a continuous oligomer at the site of DNA melting, extending from a dsDNA anchor to engage a single DNA strand. Within this complex, two nucleobases of each ssDNA binding motif (DnaA-trio) are captured within a dinucleotide binding pocket created by adjacent DnaA proteins. These results provide a molecular basis for DnaA specifically engaging the conserved sequence elements within the bacterial chromosome origin basal unwinding system (BUS).
Here, the authors show that a DnaA:origin complex promotes specific nucleobase capture from a single DNA strand. It is proposed that this mechanism may play a key role stimulating opening of bacterial chromosome origins.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 82/16
/ 82/29
/ 82/80
/ 82/83
/ Bacteria
/ Bacterial Proteins - metabolism
/ Binding
/ Chromosomes, Bacterial - genetics
/ Chromosomes, Bacterial - metabolism
/ DNA
/ DNA, Single-Stranded - genetics
/ DNA-Binding Proteins - metabolism
/ Genomes
/ Humanities and Social Sciences
/ Origins
/ Proteins
/ Science
