Journal Article

Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation

Knight, H. (University of Oxford, Oxford, UK.), Trewavas, A.J, Knight, M.R

1996

Overview

Cold shock elicits an immediate rise in cytosolic tree calcium concentration ([Ca2+]cyt) in both chilling-resistant Arabidopsis and chilling-sensitive tobacco (Nicotiana plumbaginifolia). In Arabidopsis, lanthanum or EGTA caused a partial inhibition of both cold shock [Ca2+]cyt elevation and cold-dependent kin1 gene expression. This suggested that calcium influx plays a major role in the cold shock [Ca2+]cyt response and that an intracellular calcium source also might be involved. To investigate whether the vacuole (the major intracellular calcium store in plants) is involved, we targeted the calcium-dependent photoprotein aequorin to the cytosolic face of the vacuolar membrane. Cold shock calcium kinetics in this microdomain were consistent with a cold-induced vacuolar release of calcium. Treatment with neomycin or lithium, which interferes with phosphoinositide cycling, resulted in cold shock [Ca2+]cyt kinetics consistent with the involvement of inositol trisphosphate and inositide phosphate signaling in this response. We also investigated the effects of repeated and prolonged low temperature on cold shock [Ca2+]cyt. Differences were observed between the responses of Arabidopsis and N. plumbaginifolia to repeated cold stimulation. Acclimation of Arabidopsis by pretreatment with cold or hydrogen peroxide caused a modified calcium signature to subsequent cold shock. This suggests that acclimation involves modification of plant calcium signaling to provide a \"cold memory\"

• Publisher: American Society of Plant Physiologists
• Subject: acclimation / Acclimatization / ADAPTACION / ADAPTATION / Aequorin / Aequorin - biosynthesis / Aequorin - genetics / Apoproteins / Apoproteins - biosynthesis / Apoproteins - genetics / Arabidopsis / Arabidopsis - physiology / Arabidopsis Proteins / ARABIDOPSIS THALIANA / ARN MENSAJERO / ARN MESSAGER / Base Sequence / BIOCHIMIE / BIOQUIMICA / biosynthesis / CALCIO / CALCIUM / Calcium - metabolism / CATION / CATIONES / CHELATEUR / chelating agents / CITOPLASMA / Cold shock response / cold stress / Cold Temperature / Complementary DNA / cytochemistry / CYTOPLASME / cytosol / DNA Primers / drug effects / Egtazic Acid / Egtazic Acid - pharmacology / ELEMENTOS DE TIERRAS RARAS / ethylene glycol tetraacetic acid / EXPRESION GENICA / EXPRESSION DES GENES / FOSFOLIPIDOS / FRIO / FROID / GENE / gene expression / Gene Expression Regulation, Plant / Gene Expression Regulation, Plant - drug effects / GENES / Genes, Plant / genetics / Hydrogen / INHIBICION / INHIBITION / inorganic ions / Intracellular Membranes / Intracellular Membranes - metabolism / ION / ion transport / IONES / Kinetics / lanthanum / Lanthanum - pharmacology / lipid metabolism / messenger RNA / metabolism / METABOLISME DES LIPIDES / METABOLISMO DE LIPIDOS / METAL DES TERRES RARES / Molecular Sequence Data / NICOTIANA / Nicotiana - physiology / Nicotiana plumbaginifolia / Peroxides / pharmacology / PHOSPHATIDE / phosphatidylinositols / Phosphoproteins / Phosphoproteins - biosynthesis / Phosphoproteins - genetics / physiology / Plant cells / Plant Proteins / Plant Proteins - biosynthesis / Plant Proteins - genetics / PLANTAS TRANSGENICAS / PLANTE TRANSGENIQUE / Plants / Plants, Genetically Modified / Plants, Toxic / Protein Serine-Threonine Kinases / Protein Serine-Threonine Kinases - biosynthesis / Protein Serine-Threonine Kinases - genetics / QUELATOS / Recombinant Proteins / Recombinant Proteins - biosynthesis / Recombinant Proteins - genetics / Seedlings / Signal Transduction / Signal Transduction - drug effects / structural genes / Tobacco / transgenic plants / VACUOLA / VACUOLE / Vacuoles / Vacuoles - metabolism